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Literature summary for 3.1.4.1 extracted from

  • Lerch, B.; Wolf, G.
    Isolation of phosphodiesterase from sugar beet leaves (1972), Biochim. Biophys. Acta, 258, 206-218.
    View publication on PubMed
Search for more literature for 3.1.4.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Beta vulgaris
5'-deoxyribonucleotides end-product inhibition Beta vulgaris
5'-nucleotides end-product inhibition Beta vulgaris
5'-ribonucleotides end-product inhibition Beta vulgaris
AMP product inhibition Beta vulgaris
ascorbic acid slight inhibition Beta vulgaris
EDTA
-
 Beta vulgaris
glutathione
-
 Beta vulgaris
Hg2+
-
 Beta vulgaris
L-cysteine
-
 Beta vulgaris
Reducing agents
-
 Beta vulgaris
terminal 3'-monophosphoryl group of substrates
-
 Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 4-nitrophenyl uridine 5'-phosphate
-
 Beta vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Beta vulgaris
Co2+ activation Beta vulgaris
Cu2+ activation Beta vulgaris
divalent cations activation Beta vulgaris
Mg2+ slight activation Beta vulgaris
Mn2+ activation Beta vulgaris
Zn2+ activation Beta vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Beta vulgaris

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
 var. saccharifera, sugar beet
-

Purification (Commentary)

Purification (Comment) Organism
-
 Beta vulgaris

Reaction

Reaction Comment Organism Reaction ID
[nucleotide]n + H2O = [nucleotide]m + nucleotide enzyme is an exonuclease which needs a free end of an oligonucleotide chain for activity Beta vulgaris
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
125.2
-
-
 Beta vulgaris

Storage Stability

Storage Stability Organism
frozen, several months without loss of activity Beta vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl uridine 5'-phosphate + H2O
-
 Beta vulgaris 4-nitrophenol + uridine 5'-phosphate
-
 ?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
-
 Beta vulgaris 5'-dTMP + p-nitrophenol
-
 ?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
-
 Beta vulgaris nucleoside 5'-monophosphate + p-nitrophenol
-
 ?
ADP + H2O
-
 Beta vulgaris AMP + phosphate
-
 ?
ATP + H2O
-
 Beta vulgaris 5'-AMP + diphosphate
-
 ?
DNA + H2O denatured DNA. Native DNA is resistant Beta vulgaris DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
additional information no activity with the cyclic trimer of deoxythymidine 5'-phosphate and adenosine 3',5'-monophosphate Beta vulgaris ?
-
 ?
NAD+ + H2O
-
 Beta vulgaris 5'-AMP + NMN
-
 ?
oligonucleotides + H2O
-
 Beta vulgaris oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
p-nitrophenylthymidine 5'-phosphate + H2O
-
 Beta vulgaris 5'-TMP + p-nitrophenol
-
 ?
poly A + H2O
-
 Beta vulgaris 5'-AMP
-
 ?
poly C + H2O
-
 Beta vulgaris 5'-CMP
-
 ?
poly U + H2O
-
 Beta vulgaris 5'-UMP
-
 ?
poly(I) + H2O
-
 Beta vulgaris 5'-IMP
-
 ?
polynucleotides + H2O
-
 Beta vulgaris polynucleotides(n-1) + 5'-mononucleotides
-
 ?
RNA + H2O
-
 Beta vulgaris RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 up to Beta vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
 Beta vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 above, loss in activity below pH 5 Beta vulgaris
7.9 10.5
-
 Beta vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 inactivation below, unstable near isoelectric point of 3.85 Beta vulgaris
9
-
 stable at Beta vulgaris