Any feedback?
Literature summary for 3.1.4.1 extracted from
- A new phosphodiesterase forming nucleoside 5'-monophosphate from rat liver. Its partial purification and substrate specificity for nicotinamide adenine dinucleotide and oligonucleotides (1967), J. Biol. Chem., 242, 5301-5307.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Rattus norvegicus |  |
| 5'-nucleotides | - |
Rattus norvegicus | |
| ADP | - |
Rattus norvegicus | |
| AMP | 5'-AMP and slightly 3'-AMP | Rattus norvegicus | |
| ATP | - |
Rattus norvegicus | |
| Cu2+ | - |
Rattus norvegicus | |
| CuCl2 | - |
Rattus norvegicus | |
| EDTA | - |
Rattus norvegicus | |
| Hg2+ | - |
Rattus norvegicus | |
| HgCl2 | - |
Rattus norvegicus | |
| NAD+ | competitive | Rattus norvegicus | |
| nucleoside 5'monophosphate | - |
Rattus norvegicus | |
| nucleoside-5'-diphosphate | - |
Rattus norvegicus | |
| p-chloromercuribenzoate | slight inhibition | Rattus norvegicus | |
| RNA | competitive | Rattus norvegicus | |
| terminal 3'-monophosphoryl group of substrates | - |
Rattus norvegicus | |
| UDP | - |
Rattus norvegicus | |
| UMP | 5'-UMP and very slightly 3'-UMP | Rattus norvegicus | |
| UTP | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
4-nitrophenyl uridine 5'-phosphate | - |
Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Rattus norvegicus | |
| Co2+ | slight activation | Rattus norvegicus | |
| divalent cations | activation | Rattus norvegicus | |
| K+ | activation with high concentration | Rattus norvegicus | |
| KCl | activation | Rattus norvegicus | |
| Mg2+ | activation | Rattus norvegicus | |
| monovalent cations | activation with high concentrations | Rattus norvegicus | |
| Na+ | activation with high concentration | Rattus norvegicus | |
| NaCl | activation | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.48 | - |
substrate: NAD+ | Rattus norvegicus |
| 1.2 | - |
substrate: p-nitrophenyluridine 5'-phosphate | Rattus norvegicus |
| 1.88 | - |
substrate: ApU | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl uridine 5'-phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + uridine 5'-phosphate | - |
? | |
| 5'-p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 5'-p-nitrophenylnucleoside 5'-monophosphate + H2O | - |
Rattus norvegicus | nucleoside 5'-monophosphate + p-nitrophenol | - |
? | |
| 5'-p-nitrophenyluridine 5'-phosphate + H2O | - |
Rattus norvegicus | 5'-UMP + p-nitrophenol | - |
? | |
| dinucleoside monophosphates + H2O | - |
Rattus norvegicus | mononucleoside 5'-phosphate | - |
? | |
| DNA + H2O | heat-denatured: very slowly, native DNA is not hydrolyzed | Rattus norvegicus | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
| additional information | no activity with dinucleotides bearing 3'-phosphate | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with UTP and UDP | Rattus norvegicus | ? | - |
? | |
| NAD+ + H2O | - |
Rattus norvegicus | 5'-AMP + NMN | - |
? | |
| oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Rattus norvegicus | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
| polymer synthesized from NAD+ + H2O | chain length 27-30 | Rattus norvegicus | ? | - |
? | |
| polynucleotides + H2O | very slowly | Rattus norvegicus | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
| RNA + H2O | very slowly | Rattus norvegicus | RNA(n-1) + nucleoside 5'-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme is heat labile | Rattus norvegicus |
| 50 | - |
below, enzyme is heat labile | Rattus norvegicus |
| 70 | - |
pH 7.3, 5 min, complete loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 10.5 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | - |
Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
