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Literature summary for 3.1.31.1 extracted from

  • Li, L.; Arnosti, D.
    Fine mapping of chromatin structure in Drosophila melanogaster embryos using micrococcal nuclease (2010), Fly, 4, 213-215.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.31.1

Application

Application Comment Organism
analysis the enzyme is a useful tool for mapping chromatin structure in eukaryotes, usage of the enzyme to determine the positions of nucleosomes within a region of DNA to identify dynamic changes induced during gene regulation Staphylococcus aureus
molecular biology the enzyme is a useful tool for mapping chromatin structure in eukaryotes, usage of the enzyme to determine the positions of nucleosomes within a region of DNA to identify dynamic changes induced during gene regulation Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus aureus micrococcal nuclease induces double-strand breaks within nucleosome linker regions, and with more extensive digestion, single-strand nicks within the nucleosome itself ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Staphylococcus aureus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information micrococcal nuclease induces double-strand breaks within nucleosome linker regions, and with more extensive digestion, single-strand nicks within the nucleosome itself Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
Micrococcal nuclease
-
 Staphylococcus aureus
MN ase
-
 Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Staphylococcus aureus

General Information

General Information Comment Organism
additional information MNase mapping of the enhancer chromatin structure in the Drosophila melanogaster embryo to analyse chromatin structure in a developmental setting, and identification of structural changes on a cis-regulatory element targeted by the Knirps repressor, method development and optimization, overview Staphylococcus aureus