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Literature summary for 3.1.31.1 extracted from

  • Liu, P.; Meng, X.; Qu, P.; Zhao, X.S.; Wang, C.C.
    Subdomain-specific collapse of denatured staphylococcal nuclease revealed by single molecule fluorescence resonance energy transfer measurements (2009), J. Phys. Chem. B, 113, 12030-12036.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild type enzyme and the mutants K28C/H124C K28C/K97C are expressed in BL21 (DE3) and K28C/K97C in BL21 (DE3) Escherichia coli cells Staphylococcus sp.

Protein Variants

Protein Variants Comment Organism
K28C/H124C generated by site-directed mutagenesis, native and non-native conformations are observed, and the non-native conformation expands with increasing guanidinium hydrochloride concentrations, the non-native chains of the derivative exhibits different changes of persistence length at higher guanidinium hydrochloride concentrations, suggesting a subdomain-specific collapse of the denatured state of SNase, this local chain specific collapse is likely to play a role in modulating the formation of early intermediate during protein folding Staphylococcus sp.
K28C/K97C generated by site-directed mutagenesis, native and non-native conformations are observed, and the non-native conformation expands with increasing guanidinium hydrochloride concentrations, the non-native chains of the derivative exhibits different changes of persistence length at higher guanidinium hydrochloride concentrations, suggesting a subdomain-specific collapse of the denatured state of SNase, this local chain specific collapse is likely to play a role in modulating the formation of early intermediate during protein folding Staphylococcus sp.

General Stability

General Stability Organism
denaturation of staphylococcal nuclease is induced by guanidinium hydrochloride, wild type and unlabeled mutant proteins are denatured in 20 mM Tris-HCl containing 0.1 M NaCl, pH 7.6 and 10 mM CaCl2, containing various concentrations of guanidinium hydrochloride at 25°C for 20 h to reach equilibrium Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type and mutant enzymes are purified by a chelating-sepharose fast flow column Staphylococcus sp.

Synonyms

Synonyms Comment Organism
SNase
-
Staphylococcus sp.
staphylococcal nuclease
-
Staphylococcus sp.