Crystallization (Comment) | Organism |
---|---|
crystals of the I92E and I92K variant proteins are obtained at 4°C using the hanging-drop vapor-diffusion method | Staphylococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
I92E | wild-type enzyme exhibits a broad range of pH-independence from pH 4.5 to pH 9, mutant enzyme exhibits pronounced pH-dependence with a maximal stability at pH 4.9 | Staphylococcus sp. |
I92K | wild-type enzyme exhibits a broad range of pH-independence from pH 4.5 to pH 9.0, mutant enzyme exhibits pronounced pH-dependence with a maximal stability at pH 9.8 | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
- |
- |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | 25°C, wild-type enzyme is stable in the range | Staphylococcus sp. |
4.8 | - |
25°C, maximal stability of mutant enzyme I92E | Staphylococcus sp. |
9.8 | - |
25°C, maximal stability of mutant enzyme I92K | Staphylococcus sp. |