Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.30.2 extracted from

  • Mandal, P.; Chakraborty, P.; Sau, S.; Mandal, N.C.
    Purification and characterization of a deoxyriboendonuclease from Mycobacterium smegmatis (2006), J. Biochem. Mol. Biol., 39, 140-144.
    View publication on PubMed
Search for more literature for 3.1.30.2

General Stability

General Stability Organism
addition of 10 mM EDTA essential for storage stability Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 90% inhibition at 2.5 mM, inhibition fully reversible by addition of Mg2+ Mycolicibacterium smegmatis
K+
-
 Mycolicibacterium smegmatis
additional information no inhibition by Na+ Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum activity in the presence of 2.5 mM Mycolicibacterium smegmatis
Mn2+ can replace Mg2+, more effective than Mg2+ Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Mycolicibacterium smegmatis
-
 ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends ?
dsDNA + H2O Mycolicibacterium smegmatis physiological function currently unknown ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to near homogeneity Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.315
-
 purified enzyme Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, 15 days storage on ice, fully stable for at least 15 days Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O tritium labeled DNA from Escherichia coli, fragmented by limited sonication Mycolicibacterium smegmatis ? random DNA cleavage with respect to all possible phosphodiester bonds ?
dsDNA + H2O
-
 Mycolicibacterium smegmatis ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends ?
dsDNA + H2O physiological function currently unknown Mycolicibacterium smegmatis ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds ?
lambda DNA + H2O
-
 Mycolicibacterium smegmatis ? size of linear DNA fragments decreases with prolonged incubation time ?
pUC18 DNA + H2O covalently closed circular plasmid DNA Mycolicibacterium smegmatis ? substrate converted into relaxed circular form and than to the linear form ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
 Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Mycolicibacterium smegmatis