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Literature summary for 3.1.30.2 extracted from
- Purification, properties and specificity of an endonuclease from Agropyron elongatum seedlings (2004), Plant Physiol. Biochem., 42, 795-802.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50% inhibition | Thinopyrum elongatum |  |
| Cu2+ | slight inhibition | Thinopyrum elongatum | |
| DTT | slight inhibition | Thinopyrum elongatum | |
| Mn2+ | slight inhibition | Thinopyrum elongatum | |
| additional information | no inhibition by NEM and iodoacetic acid | Thinopyrum elongatum | |
| NaCl | slight inhibition | Thinopyrum elongatum | |
| Ni2+ | slight inhibition | Thinopyrum elongatum | |
| putrescine | - |
Thinopyrum elongatum | |
| spermidine | - |
Thinopyrum elongatum | |
| spermine | 70% inhibition at 0.1 mM | Thinopyrum elongatum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by Mg2+ | Thinopyrum elongatum | |
| Zn2+ | stimulates | Thinopyrum elongatum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
1 * 34000, SDS-PAGE | Thinopyrum elongatum |
| 36000 | - |
gel filtration | Thinopyrum elongatum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsDNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids | ? | - |
? | |
| additional information | Thinopyrum elongatum | substrate specificity in descending order is ssDNA, dsDNA, RNA | ? | - |
? | |
| RNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids | ? | - |
? | |
| ssDNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids, preference for ssDNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thinopyrum elongatum | - |
8x = Elytrigia turcica | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 265fold to homogeneity from seedlings by ammonium sulfate fractionation, anion exchange chromatography and heparin affinity chromatography | Thinopyrum elongatum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Thinopyrum elongatum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified enzyme | Thinopyrum elongatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-CCTGGCAGTT + H2O | synthetic labeled deoxydecanucleotide, cleavage of 5'-Ap*G bond and with lower activity of Gp*T, Cp*A, and Gp*G bonds | Thinopyrum elongatum | ? | - |
? | |
| dsDNA + H2O | hydrolysis of nucleic acids | Thinopyrum elongatum | ? | - |
? | |
| additional information | substrate specificity in descending order is ssDNA, dsDNA, RNA | Thinopyrum elongatum | ? | - |
? | |
| additional information | substrate specificity, no activity with dinucleoside monophosphates, overview | Thinopyrum elongatum | ? | - |
? | |
| poly(I) + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
| polyA + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
| polyC + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
| polyG + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
| polyU + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
| RNA + H2O | hydrolysis of nucleic acids | Thinopyrum elongatum | ? | - |
? | |
| ssDNA + H2O | hydrolysis of nucleic acids, preference for ssDNA | Thinopyrum elongatum | ? | - |
? | |
| supercoiled plasmid DNA + H2O | - |
Thinopyrum elongatum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Thinopyrum elongatum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endonuclease | - |
Thinopyrum elongatum |
| nuclease A | - |
Thinopyrum elongatum |
| plant type I nuclease | - |
Thinopyrum elongatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Thinopyrum elongatum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, loss of 30% of activity | Thinopyrum elongatum |
| 70 | - |
10 min, loss of 90% of activity | Thinopyrum elongatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thinopyrum elongatum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thinopyrum elongatum | isoelectric focusing | - |
4.7 |
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