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Literature summary for 3.1.30.2 extracted from

  • Shlyapnikov, S.V.; Lunin, V.V.; Blagova, E.V.; Abaturov, L.V.; Perbandt, M.; Betzel, C.; Mikhailov, A.M.
    A comparative structure-function analysis and molecular mechanism of action of endonucleases from Serratia marcescens and Physarum polycephalum (2002), Russ. J. Bioorg. Chem., 28, 20-27.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ coordinates 5 water molecules that participate in the enzyme-controlled reaction Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products general acid-base catalysis. The enzyme cleaves 3'- and 5'-phosphodiester bonds to form 5'-phosphorylated oligonucleotides. The hydrolysis of the phosphodiester bonds proceeds as a result of the nucleophilic displacement at the phosphorus atom. The enzyme active site is formed at least by 8 amino acid residues, Arg57, Asp86, His89, Gln114, Asn119 and Glu127. Arg57, Asp86, His89 and Glu127 are important for the catalytic activity of the enzyme. His89 is a potential candidate for the function of a general base catalyst that accepts a proton from a solvent H2O molecule, which is the attacking nucleophile. The reaction is accompanied by the inversion of configuration at the phosphorus atom and proceeds in accordance with an in-line mechanism Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O
-
Serratia marcescens ?
-
?
dsRNA + H2O
-
Serratia marcescens ?
-
?

Synonyms

Synonyms Comment Organism
Sma
-
Serratia marcescens