Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | coordinates 5 water molecules that participate in the enzyme-controlled reaction | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products | general acid-base catalysis. The enzyme cleaves 3'- and 5'-phosphodiester bonds to form 5'-phosphorylated oligonucleotides. The hydrolysis of the phosphodiester bonds proceeds as a result of the nucleophilic displacement at the phosphorus atom. The enzyme active site is formed at least by 8 amino acid residues, Arg57, Asp86, His89, Gln114, Asn119 and Glu127. Arg57, Asp86, His89 and Glu127 are important for the catalytic activity of the enzyme. His89 is a potential candidate for the function of a general base catalyst that accepts a proton from a solvent H2O molecule, which is the attacking nucleophile. The reaction is accompanied by the inversion of configuration at the phosphorus atom and proceeds in accordance with an in-line mechanism | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + H2O | - |
Serratia marcescens | ? | - |
? | |
dsRNA + H2O | - |
Serratia marcescens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sma | - |
Serratia marcescens |