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Literature summary for 3.1.30.2 extracted from
- Purification and characterization of the 2nd 5'-nucleotide-forming nuclease from Lentinus edodes (1995), Biosci. Biotechnol. Biochem., 59, 1169-1171.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Lentinula edodes |  |
| EDTA | - |
Lentinula edodes | |
| Hg2+ | - |
Lentinula edodes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | reactivates after EDTA treatment | Lentinula edodes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Lentinula edodes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentinula edodes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nuclease Le3 | Lentinula edodes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Lentinula edodes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-nucleotides + H2O | e.g. 3'-AMP | Lentinula edodes | ? | - |
? | |
| DNA + H2O | heat denatured | Lentinula edodes | 5'-phosphonucleotides + 5'-phosphomononucleotides | - |
? | |
| RNA + H2O | - |
Lentinula edodes | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Lentinula edodes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Lentinula edodes |
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