Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.30.2 extracted from

  • Pietrzak, M.; Cudny, H.; Maluszynski, M.
    Purification and properties of two ribonucleases and a nuclease from barley seeds (1980), Biochim. Biophys. Acta, 614, 102-112.
    View publication on PubMed
Search for more literature for 3.1.30.2

Inhibitors

Inhibitors Comment Organism Structure
Co2+ slight Hordeum vulgare
Cu2+
-
 Hordeum vulgare
DNA inhibits activity against RNA Hordeum vulgare
Fe2+ slight Hordeum vulgare
Hg2+
-
 Hordeum vulgare
Mn2+ slight Hordeum vulgare
Zn2+ slight Hordeum vulgare

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ inhibition Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 barley
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O hydrolysis rate about 3 times more slowly than compared to RNA Hordeum vulgare 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
RNA + H2O polyguanylic acid Hordeum vulgare 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 single-stranded DNA Hordeum vulgare
5.8
-
 RNA Hordeum vulgare
6.8
-
-
 Hordeum vulgare
7.8
-
 3'-AMP Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7.2 pH 5.2: about 15% of activity maximum, pH 7.2: about 70% of activity maximum Hordeum vulgare