Application | Comment | Organism |
---|---|---|
molecular biology | a combination of S1 nuclease with two strands of pseudo-complementary peptide nucleic acid is useful for cleaving genomic DNA into desired fragments at targeted sites. The DNA analogue pseudo-complementary peptide nucleic acid, pcPNA, has a poly[N-aminoethylglycine] backbone and invades double-stranded DNA through Watson-Crick base-pairing. Through the invasion, single-stranded portions are formed at targeted sites in the genome and cut by the enzyme which hydrolyses only single-stranded DNA. The enzyme's mismatch-recognition activity was high enough to distinguish one base-pair difference in the invasion site. Enzyme cofactor Zn2+ suppresses the DNA invasion by pseudo-complementary peptide nucleic acid | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, divalent metal ion stabilizes the DNA duplex and suppresses the pcPNA invasion | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus oryzae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme cleaves human genomic DNA from the genome of K562 or HEK-293 cells at targeted sites by combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid, DNA fragment preparation, overview | Aspergillus oryzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S1 nuclease | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
higher activity at 50°C compared to 37°C | Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aspergillus oryzae |