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Literature summary for 3.1.30.1 extracted from

  • Li, X.; Muneoka, S.; Shigi, N.; Sumaoka, J.; Komiyama, M.
    Clipping of predetermined fragments from the human genome by S1 nuclease-PNA combinations (2014), Chem. Commun. (Camb. ), 50, 8674-8676.
    View publication on PubMed

Application

Application Comment Organism
molecular biology a combination of S1 nuclease with two strands of pseudo-complementary peptide nucleic acid is useful for cleaving genomic DNA into desired fragments at targeted sites. The DNA analogue pseudo-complementary peptide nucleic acid, pcPNA, has a poly[N-aminoethylglycine] backbone and invades double-stranded DNA through Watson-Crick base-pairing. Through the invasion, single-stranded portions are formed at targeted sites in the genome and cut by the enzyme which hydrolyses only single-stranded DNA. The enzyme's mismatch-recognition activity was high enough to distinguish one base-pair difference in the invasion site. Enzyme cofactor Zn2+ suppresses the DNA invasion by pseudo-complementary peptide nucleic acid Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, divalent metal ion stabilizes the DNA duplex and suppresses the pcPNA invasion Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Aspergillus oryzae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleaves human genomic DNA from the genome of K562 or HEK-293 cells at targeted sites by combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid, DNA fragment preparation, overview Aspergillus oryzae ?
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?

Synonyms

Synonyms Comment Organism
S1 nuclease
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Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
higher activity at 50°C compared to 37°C Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 assay at Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Aspergillus oryzae