Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia phage T7 |
Crystallization (Comment) | Organism |
---|---|
crystal structure at 2.1 A resolution | Escherichia phage T7 |
Protein Variants | Comment | Organism |
---|---|---|
D55N | similar binding affinity for DNA as wild-type, approx. 0.02% of wild-type DNA cleavage rate | Escherichia phage T7 |
D74A | lower binding affinity for DNA as wild-type, approx. 15% of wild-type DNA cleavage rate | Escherichia phage T7 |
D74N | lower binding affinity for DNA as wild-type, approx. 30% of wild-type DNA cleavage rate | Escherichia phage T7 |
E20A | lower binding affinity for DNA as wild-type, approx. 0.5% of wild-type DNA cleavage rate | Escherichia phage T7 |
E20Q | 3fold lower binding affinity for DNA as wild-type, approx. 0.1% of wild-type DNA cleavage rate | Escherichia phage T7 |
E35K | lower binding affinity for DNA as wild-type, slightly lower rate of DNA cleavage | Escherichia phage T7 |
E65K | similar binding affinity for DNA as wild-type, approx. 0.02% of wild-type DNA cleavage rate | Escherichia phage T7 |
E65Q | slightly higher binding affinity for DNA as wild-type, approx. 1% of wild-type DNA cleavage rate | Escherichia phage T7 |
K67A | lower binding affinity for DNA as wild-type, approx. 0.3% of wild-type DNA cleavage rate | Escherichia phage T7 |
K67R | 7fold lower binding affinity for DNA as wild-type, approx. 0.01% of wild-type DNA cleavage rate | Escherichia phage T7 |
P54L | lower binding affinity for DNA as wild-type, approx. 0.5% of wild-type DNA cleavage rate | Escherichia phage T7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia phage T7 | - |
nicked DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant T7 endonuclease I | Escherichia phage T7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Escherichia phage T7 | nicked DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T7 endonuclease I | - |
Escherichia phage T7 |