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Literature summary for 3.1.30.1 extracted from

  • Declais, A.C.; Hadden, J.; Phillips, S.E.V.; Lilley, D.M.J.
    The active site of the junction-resolving enzyme T7 endonuclease I (2001), J. Mol. Biol., 307, 1145-1158.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia phage T7

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure at 2.1 A resolution Escherichia phage T7

Protein Variants

Protein Variants Comment Organism
D55N similar binding affinity for DNA as wild-type, approx. 0.02% of wild-type DNA cleavage rate Escherichia phage T7
D74A lower binding affinity for DNA as wild-type, approx. 15% of wild-type DNA cleavage rate Escherichia phage T7
D74N lower binding affinity for DNA as wild-type, approx. 30% of wild-type DNA cleavage rate Escherichia phage T7
E20A lower binding affinity for DNA as wild-type, approx. 0.5% of wild-type DNA cleavage rate Escherichia phage T7
E20Q 3fold lower binding affinity for DNA as wild-type, approx. 0.1% of wild-type DNA cleavage rate Escherichia phage T7
E35K lower binding affinity for DNA as wild-type, slightly lower rate of DNA cleavage Escherichia phage T7
E65K similar binding affinity for DNA as wild-type, approx. 0.02% of wild-type DNA cleavage rate Escherichia phage T7
E65Q slightly higher binding affinity for DNA as wild-type, approx. 1% of wild-type DNA cleavage rate Escherichia phage T7
K67A lower binding affinity for DNA as wild-type, approx. 0.3% of wild-type DNA cleavage rate Escherichia phage T7
K67R 7fold lower binding affinity for DNA as wild-type, approx. 0.01% of wild-type DNA cleavage rate Escherichia phage T7
P54L lower binding affinity for DNA as wild-type, approx. 0.5% of wild-type DNA cleavage rate Escherichia phage T7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia phage T7
-
nicked DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant T7 endonuclease I Escherichia phage T7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Escherichia phage T7 nicked DNA
-
?

Synonyms

Synonyms Comment Organism
T7 endonuclease I
-
Escherichia phage T7