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Literature summary for 3.1.30.1 extracted from
- Single-strand-specific nucleases (2003), FEMS Microbiol. Rev., 26, 457-491.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal activity at 100 mM | Aspergillus oryzae |  |
General Stability
| General Stability | Organism |
|---|---|
| no loss of activity in the presence of 100-250 mM glyoxal | Aspergillus oryzae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus oryzae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc metalloprotein, required for activity | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000 | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | Aspergillus oryzae | - |
fragments of RNA | - |
? | |
| ssDNA + H2O | Aspergillus oryzae | - |
fragments of ssDNA | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dimethyl sulfoxide | no loss of activity in 50% dimethylsulfoxide | Aspergillus oryzae |
| dimethylformamide | no loss of activity in 30% dimethylformamide | Aspergillus oryzae |
| formaldehyde | no loss of activity in 2% formaldehyde | Aspergillus oryzae |
| formamide | no loss of activity in 60% formamide | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 18% carbohydrates | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | - |
Aspergillus oryzae | fragments of RNA | - |
? | |
| ssDNA + H2O | - |
Aspergillus oryzae | fragments of ssDNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000 | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S1 nuclease | - |
Aspergillus oryzae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
4 |
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Release 2023.1 (January 2023)
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