BRENDA - Enzyme Database
show all sequences of 3.1.30.1

Purification and characterization of a DNA single strand specific endonuclease from human cells

Wang, E.C.; Furth, J.J.; Rose, J.A.; Biochemistry 17, 544-549 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
Cu2+
46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
Fe2+
47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
Hg2+
31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
Mn2+
6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
N-ethylmaleimide
with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
Homo sapiens
NaCl
inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+
Homo sapiens
Zn2+
33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
denaturated DNA
-
Homo sapiens
0.02
-
polydeoxythymidylic acid
-
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
DNA + H2O
Homo sapiens
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Homo sapiens
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
KB cell
pharyngal carcinoma cell line
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
purified enzyme, polydeoxythymidylic acid as substrate
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
DNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
dsDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxyadenylic acid + H2O
-
134996
Homo sapiens
5'-dAMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxycytosylic acid + H2O
only little degradation
134996
Homo sapiens
5'-dCMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxyguanylic acid + H2O
-
134996
Homo sapiens
5'-dGMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxythymidylic acid + H2O
-
134996
Homo sapiens
5'-dTMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polyuridylic acid + H2O
80% of activity with polydeoxythymidylic acid as substrate
134996
Homo sapiens
5'-UMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
ssDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides
134996
Homo sapiens
?
Synonyms
Synonyms
Commentary
Organism
KB endonuclease
-
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Homo sapiens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.2
-
with denaturated DNA and polydeoxythymidylic acid as substrate
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
10.5
12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
Cu2+
46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
Fe2+
47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
Hg2+
31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
Mn2+
6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
N-ethylmaleimide
with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
Homo sapiens
NaCl
inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+
Homo sapiens
Zn2+
33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
denaturated DNA
-
Homo sapiens
0.02
-
polydeoxythymidylic acid
-
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
DNA + H2O
Homo sapiens
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Homo sapiens
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
KB cell
pharyngal carcinoma cell line
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
purified enzyme, polydeoxythymidylic acid as substrate
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
DNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
dsDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxyadenylic acid + H2O
-
134996
Homo sapiens
5'-dAMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxycytosylic acid + H2O
only little degradation
134996
Homo sapiens
5'-dCMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxyguanylic acid + H2O
-
134996
Homo sapiens
5'-dGMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polydeoxythymidylic acid + H2O
-
134996
Homo sapiens
5'-dTMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
polyuridylic acid + H2O
80% of activity with polydeoxythymidylic acid as substrate
134996
Homo sapiens
5'-UMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
ssDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides
134996
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Homo sapiens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.2
-
with denaturated DNA and polydeoxythymidylic acid as substrate
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
10.5
12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5
Homo sapiens
Other publictions for EC 3.1.30.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728950
Wang
Label-free fluorometric detect ...
Aspergillus oryzae
Anal. Biochem.
468
34-38
2014
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1
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729583
Li
Clipping of predetermined frag ...
Aspergillus oryzae
Chem. Commun. (Camb. )
50
8674-8676
2014
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1
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730771
Guan
Label-free and sensitive fluor ...
Aspergillus oryzae
PLoS ONE
9
e108401
2014
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1
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1
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730587
Lesniewicz
The plant S1-like nuclease fam ...
Arabidopsis thaliana
Plant Cell Physiol.
54
1064-1078
2013
-
-
1
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1
2
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1
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4
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6
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1
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1
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2
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1
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6
-
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1
-
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1
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2
2
-
-
-
718072
Balabanova
An extracellular S1-type nucle ...
Penicillium melinii
Mar. Biotechnol.
14
87-95
2012
-
-
-
-
-
-
7
-
1
7
1
2
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2
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1
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1
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2
1
1
1
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7
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7
1
2
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1
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1
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1
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2
2
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-
683938
Sasaki
Regulation of DNA nucleases by ...
Aspergillus oryzae
Nucleic Acids Res.
35
4086-4093
2007
1
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663897
Pimkin
Characterization of a periplas ...
Mesorhizobium loti
Biochem. Biophys. Res. Commun.
343
77-84
2006
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1
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3
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1
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5
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664133
Liu
Mechanistic aspects of the DNA ...
Escherichia phage T7
Biochemistry
45
3934-3942
2006
-
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3
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2
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683173
Bochkov
Hydrolytic approach for produc ...
Aspergillus oryzae
Biochemistry (Moscow)
71
79-83
2006
-
-
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2
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1
1
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2
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1
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683587
Tang
Direct visualization of enzyma ...
Aspergillus oryzae
J. Am. Chem. Soc.
128
14972-14976
2006
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683767
Hong
-
Effective family shuffling met ...
Aspergillus oryzae
J. Microbiol. Biotechnol.
16
2004-2007
2006
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664417
Maruyama
Mutation detection in DNA olig ...
Escherichia phage T7
Biotechnol. Lett.
27
1349-1354
2005
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1
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666502
Guan
A single catalytic domain of t ...
Escherichia phage T7
Nucleic Acids Res.
33
6225-6234
2005
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-
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654746
Guan
Changing the enzymatic activit ...
Escherichia phage T7
Biochemistry
43
4313-4322
2004
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1
1
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656882
Till
Mismatch cleavage by single-st ...
Apium graveolens, Aspergillus oryzae, Vigna radiata
Nucleic Acids Res.
32
2632-2641
2004
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1
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5
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3
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3
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3
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2
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-
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-
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-
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-
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-
3
-
-
-
-
-
-
-
-
-
666488
Ren
Straightforward detection of S ...
Aspergillus oryzae
Nucleic Acids Res.
32
e42
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655331
Declais
The complex between a four-way ...
Escherichia phage T7
EMBO J.
22
1398-1409
2003
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655642
Desai
Single-strand-specific nucleas ...
Aspergillus oryzae
FEMS Microbiol. Rev.
26
457-491
2003
1
-
-
-
-
1
-
-
1
1
1
2
4
6
-
1
-
-
-
-
-
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1
1
-
-
-
-
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-
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-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
656529
Freeman
Metal ion binding in the activ ...
Escherichia phage T7
J. Mol. Biol.
333
59-73
2003
-
-
1
1
8
-
1
-
-
4
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
1
-
-
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655322
Hosseini
-
Application of rice (Oryza sat ...
Oryza sativa
Electron. J. Biotechnol.
5
42-54
2002
-
-
-
-
-
-
5
-
-
1
1
1
-
1
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
655943
Desai
-
Nuclease Bh1 from Basidiobolus ...
Basidiobolus haptosporus
J. Biochem. Mol. Biol. Biophys.
5
267-275
2001
-
-
-
-
-
1
11
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
11
-
-
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-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
655944
Rangarajan
-
Active site characterization o ...
Rhizopus stolonifer
J. Biochem. Mol. Biol. Biophys.
5
309-325
2001
-
-
-
-
-
-
3
-
-
-
-
2
-
1
-
-
1
-
-
-
-
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-
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-
-
-
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-
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-
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-
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1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655945
Desai
-
Active site characterization o ...
Basidiobolus haptosporus
J. Biochem. Mol. Biol. Biophys.
5
327-334
2001
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656487
Declais
The active site of the junctio ...
Escherichia phage T7
J. Mol. Biol.
307
1145-1158
2001
-
-
1
1
11
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
11
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655494
Schneider
Comparative analysis of follis ...
Aspergillus oryzae
Eur. J. Endocrinol.
142
537-544
2000
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655989
Kar
The immunologically protective ...
Leishmania pifanoi
J. Biol. Chem.
275
37789-37797
2000
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135004
Johnson
Plasmid RK2 ParB protein: puri ...
Escherichia coli
J. Bacteriol.
181
6010-6018
1999
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135002
Romier
Recognition of single-stranded ...
Penicillium citrinum
Proteins
32
414-424
1998
-
-
-
-
-
-
-
-
-
1
-
2
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134128
Stoynov
Single-strand-specific DNase a ...
Crotalus adamanteus
FEBS Lett.
409
151-154
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134999
Ikeda
Identification and characteriz ...
Schizosaccharomyces pombe
Biochem. Mol. Biol. Int.
40
1017-1024
1996
-
-
-
-
-
-
5
-
1
2
1
2
-
1
-
-
1
-
-
-
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
2
1
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134982
Lee
Cloning, characterization and ...
Aspergillus oryzae
Appl. Microbiol. Biotechnol.
44
425-431
1995
-
-
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134983
Gite
S1 nuclease: immunoaffinity pu ...
Aspergillus oryzae
J. Mol. Recognit.
8
281-289
1995
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134980
Demidov
Sequence selective double stra ...
Aspergillus oryzae
Nucleic Acids Res.
21
2103-2107
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134981
Gite
Preparation, properties and ap ...
Aspergillus oryzae
Biotechnol. Appl. Biochem.
17
373-382
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
2
-
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
2
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
134978
Gite
Characterization of S1 nucleas ...
Aspergillus oryzae
Eur. J. Biochem.
210
437-441
1992
-
-
-
-
-
-
2
-
-
2
-
2
-
2
-
-
1
-
-
-
-
1
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
-
1
-
-
-
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134979
Gite
Active-site characterization o ...
Aspergillus oryzae
Biochem. J.
285
489-494
1992
-
-
-
-
-
-
3
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135000
Volbeda
Crystal structure of Penicilli ...
Penicillium citrinum
EMBO J.
10
1607-1618
1991
-
-
-
-
-
-
-
-
-
1
-
2
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135001
Maekawa
Primary structure of nuclease ...
Penicillium citrinum
Eur. J. Biochem.
200
651-661
1991
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134976
Ostrem
Purification of S1 nuclease fr ...
Aspergillus oryzae
Electrophoresis
11
953-957
1990
-
2
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134977
Butour
Kinetic studies of the hydroly ...
Aspergillus oryzae
Chem. Biol. Interact.
73
195-205
1990
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134989
Blank
Single-strand preferring nucle ...
Triticum aestivum
Proc. Natl. Acad. Sci. USA
86
3169-3173
1989
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
95076
Naseem
Single-strand-specific nucleas ...
Pisum sativum
Arch. Biochem. Biophys.
255
437-445
1987
-
-
-
-
-
-
3
-
-
2
3
1
-
2
-
1
1
-
-
2
1
-
3
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
3
1
-
-
1
1
-
2
1
-
3
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
134974
Reddy
Immobilization of single-stran ...
Aspergillus oryzae
Appl. Biochem. Biotechnol.
14
231-240
1987
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
2
3
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
3
-
1
-
1
-
1
-
-
-
-
-
-
-
134972
Shishido
Purification of S1 nuclease to ...
Aspergillus oryzae
Biochim. Biophys. Acta
884
215-218
1986
-
-
-
-
-
-
-
-
-
1
1
1
-
1
-
1
1
-
-
-
-
-
6
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
1
1
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
134973
Martin
-
A comparative study of nucleas ...
Aspergillus oryzae, Neurospora crassa, Penicillium citrinum, Physarum polycephalum, Pisum sativum, Schizophyllum commune, Triticum aestivum, Ustilago maydis, Vigna radiata var. radiata
Biochim. Biophys. Acta
867
76-80
1986
-
5
-
-
-
-
12
-
-
13
11
9
-
9
-
-
-
-
-
-
-
-
18
-
5
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
12
-
-
-
13
11
9
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134988
Martin
-
Partial purification and prope ...
Schizophyllum commune
Biochim. Biophys. Acta
867
67-75
1986
-
-
-
-
-
-
10
-
-
3
1
2
-
1
-
-
1
-
-
-
-
-
4
-
1
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
3
1
2
-
-
-
1
-
-
-
-
4
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
134991
Tomkinson
Purification and properties of ...
Mus musculus
Nucleic Acids Res.
14
9579-9583
1986
-
-
-
-
-
-
4
-
1
2
1
2
-
1
-
-
1
-
-
1
1
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
2
1
2
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134971
Shishido
-
Single-strand-specific nucleas ...
Aspergillus oryzae, Bacillus subtilis, Bacillus subtilis Marburg / DSM 10 / ATCC 6051, Escherichia coli, Homo sapiens, Mus musculus, Mustelus canis, Neurospora crassa, Penicillium citrinum, Physarum polycephalum, Pseudoalteromonas espejiana, Triticum aestivum, Ustilago maydis, Vigna radiata var. radiata
Cold Spring Harbor Monogr. Ser.
14
155-185
1982
4
6
-
-
-
-
36
-
-
21
8
22
-
16
-
3
-
-
-
4
-
1
73
-
9
-
2
3
-
8
3
2
-
-
-
-
4
6
-
-
-
-
-
-
36
-
-
-
21
8
22
-
-
3
-
-
4
-
1
73
-
-
2
3
-
8
3
2
-
-
-
-
-
-
-
134968
Silber
S1 nuclease does not cleave DN ...
Aspergillus oryzae, Neurospora crassa
Biochim. Biophys. Acta
656
256-264
1981
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134969
Lehman
-
Endonucleases specific for sin ...
Aspergillus oryzae, Neurospora crassa, Vigna radiata var. radiata
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
193-201
1981
-
3
-
-
-
-
7
-
-
5
5
6
-
3
-
2
-
-
-
2
-
-
13
-
2
-
1
-
-
2
1
-
-
1
-
-
-
3
-
-
-
-
-
-
7
1
-
-
5
5
6
-
-
2
-
-
2
-
-
13
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
134970
Oleson
S1 nuclease of Aspergillus ory ...
Aspergillus oryzae
Arch. Biochem. Biophys.
211
478-484
1981
-
-
-
-
-
-
5
11
-
-
-
1
-
2
-
-
-
-
-
-
11
-
14
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
11
-
-
-
1
-
-
-
-
-
-
11
-
14
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134994
Wani
Pisum sativum endonuclease. St ...
Pisum sativum
Biochim. Biophys. Acta
655
396-406
1981
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134967
Vogt
Purification and properties of ...
Aspergillus oryzae
Methods Enzymol.
65
248-255
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134975
Oleson
S1 nuclease of Aspergillus ory ...
Aspergillus oryzae
Arch. Biochem. Biophys.
204
361-370
1980
-
2
-
-
-
-
2
1
-
1
1
1
-
2
-
1
1
-
-
-
-
1
10
-
1
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
1
1
1
-
-
1
1
-
-
-
1
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
134984
Fraser
Purification and properties of ...
Neurospora crassa
Methods Enzymol.
65
255-263
1980
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134987
Laskowski
Purification and properties of ...
Vigna radiata var. radiata
Methods Enzymol.
65
263-276
1980
1
-
-
-
-
-
-
-
-
1
1
1
-
2
-
1
1
-
-
-
-
1
4
-
2
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
1
1
-
-
-
1
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
134998
Przykorska
Single-strand-specific nucleas ...
Secale cereale
Eur. J. Biochem.
108
285-293
1980
-
-
-
-
-
-
1
-
3
-
1
1
-
2
-
-
1
-
-
-
-
-
8
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
1
1
-
-
-
1
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134992
Wani
Partial purification and prope ...
Pisum sativum
Arch. Biochem. Biophys.
196
138-146
1979
-
-
-
-
-
-
3
-
-
2
1
1
-
1
-
-
1
-
-
1
-
-
3
-
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
1
1
-
-
-
1
-
1
-
-
3
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
134993
Waterborg
Purification of an alkaline nu ...
Physarum polycephalum
Biochim. Biophys. Acta
571
359
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134996
Wang
Purification and characterizat ...
Homo sapiens
Biochemistry
17
544-549
1978
-
-
-
-
-
-
8
2
-
1
1
1
-
1
-
-
1
-
-
1
1
-
8
-
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
2
-
1
1
1
-
-
-
1
-
1
1
-
8
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
134997
Cobianchi
Properties of the high-molecul ...
Bacillus subtilis
Eur. J. Biochem.
84
533-539
1978
2
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
134966
Zechel
Degradation of nucleic acids i ...
Aspergillus oryzae
Eur. J. Biochem.
77
133-139
1977
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
134990
Otto
An endonuclease from mouse cel ...
Mus musculus
Eur. J. Biochem.
71
617-622
1976
2
-
-
-
-
-
4
-
-
1
1
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
-
-
1
1
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
134963
Shishido
-
Action of single-strand-specif ...
Aspergillus oryzae
Agric. Biol. Chem.
39
673-681
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134964
Wiegand
Specificity of the S1 nuclease ...
Aspergillus oryzae
J. Biol. Chem.
250
8848-8855
1975
-
1
-
-
-
-
4
-
-
3
-
1
-
3
-
-
1
-
-
-
1
-
2
-
1
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
-
-
4
2
-
-
3
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
134965
Harada
Specific cleavage of tRNA by n ...
Aspergillus oryzae
Nucleic Acids Res.
2
865-871
1975
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
134985
Tal
The cleavage of transfer RNA b ...
Neurospora crassa
Nucleic Acids Res.
2
1073-1082
1975
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134986
Fraser
A second form of the single-st ...
Neurospora crassa
Can. J. Biochem.
54
971-980
1975
-
-
-
-
-
-
2
-
-
-
-
2
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
134961
Vogt
Purification and further prope ...
Aspergillus oryzae
Eur. J. Biochem.
33
192-200
1973
-
-
-
-
-
-
2
-
-
4
1
2
-
2
-
-
1
-
-
-
-
-
7
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
4
1
2
-
-
-
1
-
-
-
-
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
134962
Godson
Action of the single-stranded ...
Aspergillus oryzae
Biochim. Biophys. Acta
308
59-67
1973
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134960
Sutton
A crude nuclease preparation s ...
Aspergillus oryzae
Biochim. Biophys. Acta
240
522-531
1971
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134959
Ando
A nuclease specific for heat d ...
Aspergillus oryzae
Biochim. Biophys. Acta
114
158-168
1966
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-