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Literature summary for 3.1.30.1 extracted from

  • Wang, E.C.; Furth, J.J.; Rose, J.A.
    Purification and characterization of a DNA single strand specific endonuclease from human cells (1978), Biochemistry, 17, 544-549.
    View publication on PubMed
Search for more literature for 3.1.30.1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition Homo sapiens
Cu2+ 46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition Homo sapiens
Fe2+ 47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition Homo sapiens
Hg2+ 31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition Homo sapiens
Mn2+ 6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition Homo sapiens
N-ethylmaleimide with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid Homo sapiens
NaCl inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+ Homo sapiens
Zn2+ 33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 denaturated DNA
-
 Homo sapiens
0.02
-
 polydeoxythymidylic acid
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Homo sapiens
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
KB cell pharyngal carcinoma cell line Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 purified enzyme, polydeoxythymidylic acid as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
denatured DNA + H2O
-
 Homo sapiens ?
-
 ?
DNA + H2O
-
 Homo sapiens 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
dsDNA + H2O
-
 Homo sapiens 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
polydeoxyadenylic acid + H2O
-
 Homo sapiens 5'-dAMP + 5'-phosphooligonucleotides
-
 ?
polydeoxycytosylic acid + H2O only little degradation Homo sapiens 5'-dCMP + 5'-phosphooligonucleotides
-
 ?
polydeoxyguanylic acid + H2O
-
 Homo sapiens 5'-dGMP + 5'-phosphooligonucleotides
-
 ?
polydeoxythymidylic acid + H2O
-
 Homo sapiens 5'-dTMP + 5'-phosphooligonucleotides
-
 ?
polyuridylic acid + H2O 80% of activity with polydeoxythymidylic acid as substrate Homo sapiens 5'-UMP + 5'-phosphooligonucleotides
-
 ?
ssDNA + H2O
-
 Homo sapiens 5'-phosphomononucleotides + 5'-phosphooligonucleotides no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides ?

Synonyms

Synonyms Comment Organism
KB endonuclease
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
 with denaturated DNA and polydeoxythymidylic acid as substrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5 12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5 Homo sapiens