BRENDA - Enzyme Database
show all sequences of 3.1.30.1

Pisum sativum endonuclease. Studies on substrate specifity and possible use as a biochemical tool

Wani, A.A.; Hart, R.W.; Biochim. Biophys. Acta 655, 396-406 (1981)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NaCl
dependence of cleavage of superhelical, nicked-circular and linear DNA molecules from the NaCl concentration
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
DNA + H2O
Pisum sativum
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Pisum sativum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DNA + H2O
-
134994
Pisum sativum
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
dsDNA + H2O
cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules
134994
Pisum sativum
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
duplex deoxyadenosine-deoxythymine homopolymer + H2O
-
134994
Pisum sativum
5'-dAMP + 5'-TMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
polydeoxyadenylic acid + H2O
more slowly, but significant hydrolysis in comparision with denaturated DNA
134994
Pisum sativum
5'-dAMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
polydeoxyguanylic acid + H2O
only little degradation, no degradation in the presence of Ca2+
134994
Pisum sativum
5'-dGMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
polydeoxythymidylic acid + H2O
-
134994
Pisum sativum
5'-dTMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
ssDNA + H2O
heat denaturated, 100°C, 5-15 min
134994
Pisum sativum
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NaCl
dependence of cleavage of superhelical, nicked-circular and linear DNA molecules from the NaCl concentration
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
DNA + H2O
Pisum sativum
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Pisum sativum
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DNA + H2O
-
134994
Pisum sativum
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
dsDNA + H2O
cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules
134994
Pisum sativum
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
duplex deoxyadenosine-deoxythymine homopolymer + H2O
-
134994
Pisum sativum
5'-dAMP + 5'-TMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
polydeoxyadenylic acid + H2O
more slowly, but significant hydrolysis in comparision with denaturated DNA
134994
Pisum sativum
5'-dAMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
polydeoxyguanylic acid + H2O
only little degradation, no degradation in the presence of Ca2+
134994
Pisum sativum
5'-dGMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
polydeoxythymidylic acid + H2O
-
134994
Pisum sativum
5'-dTMP + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
ssDNA + H2O
heat denaturated, 100°C, 5-15 min
134994
Pisum sativum
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134994
Pisum sativum
?
Other publictions for EC 3.1.30.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728950
Wang
Label-free fluorometric detect ...
Aspergillus oryzae
Anal. Biochem.
468
34-38
2014
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729583
Li
Clipping of predetermined frag ...
Aspergillus oryzae
Chem. Commun. (Camb. )
50
8674-8676
2014
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730771
Guan
Label-free and sensitive fluor ...
Aspergillus oryzae
PLoS ONE
9
e108401
2014
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730587
Lesniewicz
The plant S1-like nuclease fam ...
Arabidopsis thaliana
Plant Cell Physiol.
54
1064-1078
2013
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1
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1
2
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1
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4
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6
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6
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1
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2
2
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718072
Balabanova
An extracellular S1-type nucle ...
Penicillium melinii
Mar. Biotechnol.
14
87-95
2012
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7
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1
7
1
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683938
Sasaki
Regulation of DNA nucleases by ...
Aspergillus oryzae
Nucleic Acids Res.
35
4086-4093
2007
1
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663897
Pimkin
Characterization of a periplas ...
Mesorhizobium loti
Biochem. Biophys. Res. Commun.
343
77-84
2006
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1
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3
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5
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664133
Liu
Mechanistic aspects of the DNA ...
Enterobacteria phage T7
Biochemistry
45
3934-3942
2006
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3
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683173
Bochkov
Hydrolytic approach for produc ...
Aspergillus oryzae
Biochemistry (Moscow)
71
79-83
2006
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683587
Tang
Direct visualization of enzyma ...
Aspergillus oryzae
J. Am. Chem. Soc.
128
14972-14976
2006
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683767
Hong
-
Effective family shuffling met ...
Aspergillus oryzae
J. Microbiol. Biotechnol.
16
2004-2007
2006
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664417
Maruyama
Mutation detection in DNA olig ...
Enterobacteria phage T7
Biotechnol. Lett.
27
1349-1354
2005
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666502
Guan
A single catalytic domain of t ...
Enterobacteria phage T7
Nucleic Acids Res.
33
6225-6234
2005
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654746
Guan
Changing the enzymatic activit ...
Enterobacteria phage T7
Biochemistry
43
4313-4322
2004
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656882
Till
Mismatch cleavage by single-st ...
Apium graveolens, Aspergillus oryzae, Vigna radiata
Nucleic Acids Res.
32
2632-2641
2004
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666488
Ren
Straightforward detection of S ...
Aspergillus oryzae
Nucleic Acids Res.
32
e42
2004
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655331
Declais
The complex between a four-way ...
Enterobacteria phage T7
EMBO J.
22
1398-1409
2003
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655642
Desai
Single-strand-specific nucleas ...
Aspergillus oryzae
FEMS Microbiol. Rev.
26
457-491
2003
1
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6
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656529
Freeman
Metal ion binding in the activ ...
Enterobacteria phage T7
J. Mol. Biol.
333
59-73
2003
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1
1
8
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655322
Hosseini
-
Application of rice (Oryza sat ...
Oryza sativa
Electron. J. Biotechnol.
5
42-54
2002
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5
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655943
Desai
-
Nuclease Bh1 from Basidiobolus ...
Basidiobolus haptosporus
J. Biochem. Mol. Biol. Biophys.
5
267-275
2001
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11
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11
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655944
Rangarajan
-
Active site characterization o ...
Rhizopus stolonifer
J. Biochem. Mol. Biol. Biophys.
5
309-325
2001
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Identification and characteriz ...
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Cloning, characterization and ...
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S1 nuclease: immunoaffinity pu ...
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Sequence selective double stra ...
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Preparation, properties and ap ...
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Characterization of S1 nucleas ...
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Active-site characterization o ...
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Volbeda
Crystal structure of Penicilli ...
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Maekawa
Primary structure of nuclease ...
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Ostrem
Purification of S1 nuclease fr ...
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Electrophoresis
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Butour
Kinetic studies of the hydroly ...
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Blank
Single-strand preferring nucle ...
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95076
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Single-strand-specific nucleas ...
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134974
Reddy
Immobilization of single-stran ...
Aspergillus oryzae
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231-240
1987
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1
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134972
Shishido
Purification of S1 nuclease to ...
Aspergillus oryzae
Biochim. Biophys. Acta
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215-218
1986
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6
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134973
Martin
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A comparative study of nucleas ...
Aspergillus oryzae, Neurospora crassa, Penicillium citrinum, Physarum polycephalum, Pisum sativum, Schizophyllum commune, Triticum aestivum, Ustilago maydis, Vigna radiata var. radiata
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76-80
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18
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134988
Martin
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Partial purification and prope ...
Schizophyllum commune
Biochim. Biophys. Acta
867
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1986
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10
-
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1
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4
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1
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1
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134991
Tomkinson
Purification and properties of ...
Mus musculus
Nucleic Acids Res.
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4
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1
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134971
Shishido
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Single-strand-specific nucleas ...
Aspergillus oryzae, Bacillus subtilis, Bacillus subtilis Marburg / DSM 10 / ATCC 6051, Escherichia coli, Homo sapiens, Mus musculus, Mustelus canis, Neurospora crassa, Penicillium citrinum, Physarum polycephalum, Pseudoalteromonas espejiana, Triticum aestivum, Ustilago maydis, Vigna radiata var. radiata
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73
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8
3
2
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6
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22
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1
73
-
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2
3
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8
3
2
-
-
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134968
Silber
S1 nuclease does not cleave DN ...
Aspergillus oryzae, Neurospora crassa
Biochim. Biophys. Acta
656
256-264
1981
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4
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4
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-
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134969
Lehman
-
Endonucleases specific for sin ...
Aspergillus oryzae, Neurospora crassa, Vigna radiata var. radiata
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1981
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7
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6
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3
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2
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2
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1
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2
1
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1
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3
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7
1
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5
5
6
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2
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2
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13
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1
-
-
2
1
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-
-
-
-
-
-
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134970
Oleson
S1 nuclease of Aspergillus ory ...
Aspergillus oryzae
Arch. Biochem. Biophys.
211
478-484
1981
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11
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11
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14
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1
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5
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11
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1
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11
-
14
-
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-
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1
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-
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-
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134994
Wani
Pisum sativum endonuclease. St ...
Pisum sativum
Biochim. Biophys. Acta
655
396-406
1981
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7
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-
-
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-
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-
-
-
-
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1
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1
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-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
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-
-
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-
134967
Vogt
Purification and properties of ...
Aspergillus oryzae
Methods Enzymol.
65
248-255
1980
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-
-
-
-
-
-
-
-
-
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2
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2
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1
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4
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1
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1
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2
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1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134975
Oleson
S1 nuclease of Aspergillus ory ...
Aspergillus oryzae
Arch. Biochem. Biophys.
204
361-370
1980
-
2
-
-
-
-
2
1
-
1
1
1
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2
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1
1
-
-
-
-
1
10
-
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-
1
1
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-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
1
1
1
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-
1
1
-
-
-
1
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
134984
Fraser
Purification and properties of ...
Neurospora crassa
Methods Enzymol.
65
255-263
1980
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-
-
-
-
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1
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2
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1
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1
1
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3
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1
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1
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2
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1
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134987
Laskowski
Purification and properties of ...
Vigna radiata var. radiata
Methods Enzymol.
65
263-276
1980
1
-
-
-
-
-
-
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1
1
1
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2
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1
1
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1
4
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1
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1
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1
1
1
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-
1
1
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-
-
1
4
-
-
-
-
-
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-
1
-
-
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-
-
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134998
Przykorska
Single-strand-specific nucleas ...
Secale cereale
Eur. J. Biochem.
108
285-293
1980
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1
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3
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1
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1
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8
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1
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1
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3
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1
1
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1
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-
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8
-
-
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-
-
1
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-
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134992
Wani
Partial purification and prope ...
Pisum sativum
Arch. Biochem. Biophys.
196
138-146
1979
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3
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2
1
1
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1
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1
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1
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3
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2
1
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1
1
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-
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-
-
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-
-
-
-
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3
-
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-
2
1
1
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-
1
-
1
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-
3
-
2
1
-
-
1
1
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-
-
-
-
-
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-
134993
Waterborg
Purification of an alkaline nu ...
Physarum polycephalum
Biochim. Biophys. Acta
571
359
1979
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-
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1
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3
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1
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1
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-
-
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-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134996
Wang
Purification and characterizat ...
Homo sapiens
Biochemistry
17
544-549
1978
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-
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-
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8
2
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1
1
1
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1
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1
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-
1
1
-
8
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
2
-
1
1
1
-
-
-
1
-
1
1
-
8
-
1
-
1
-
1
1
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-
-
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134997
Cobianchi
Properties of the high-molecul ...
Bacillus subtilis
Eur. J. Biochem.
84
533-539
1978
2
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1
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1
1
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1
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1
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-
-
-
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2
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
1
1
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-
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1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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134966
Zechel
Degradation of nucleic acids i ...
Aspergillus oryzae
Eur. J. Biochem.
77
133-139
1977
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1
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2
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2
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-
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3
-
1
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-
-
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-
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-
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1
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-
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2
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-
-
-
-
3
-
1
-
-
-
-
-
-
-
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-
-
-
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-
134990
Otto
An endonuclease from mouse cel ...
Mus musculus
Eur. J. Biochem.
71
617-622
1976
2
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-
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4
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1
1
1
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1
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1
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1
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2
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-
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-
1
1
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-
-
-
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2
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-
-
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4
-
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1
1
1
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-
1
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
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134963
Shishido
-
Action of single-strand-specif ...
Aspergillus oryzae
Agric. Biol. Chem.
39
673-681
1975
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1
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1
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2
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1
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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134964
Wiegand
Specificity of the S1 nuclease ...
Aspergillus oryzae
J. Biol. Chem.
250
8848-8855
1975
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1
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4
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3
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1
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3
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1
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-
-
1
-
2
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-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
-
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4
2
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-
3
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
2
-
-
-
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-
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134965
Harada
Specific cleavage of tRNA by n ...
Aspergillus oryzae
Nucleic Acids Res.
2
865-871
1975
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1
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2
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1
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-
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3
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-
1
-
-
-
-
-
-
-
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-
1
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-
-
-
-
-
-
-
-
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-
2
-
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-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
134985
Tal
The cleavage of transfer RNA b ...
Neurospora crassa
Nucleic Acids Res.
2
1073-1082
1975
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-
-
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1
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2
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1
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1
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3
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-
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-
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-
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-
-
-
-
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-
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-
-
1
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-
-
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2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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134986
Fraser
A second form of the single-st ...
Neurospora crassa
Can. J. Biochem.
54
971-980
1975
-
-
-
-
-
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2
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-
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2
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1
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1
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1
-
-
4
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
1
-
-
-
-
-
-
-
-
-
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134961
Vogt
Purification and further prope ...
Aspergillus oryzae
Eur. J. Biochem.
33
192-200
1973
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-
-
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-
2
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4
1
2
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2
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1
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-
-
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7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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4
1
2
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-
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1
-
-
-
-
7
-
-
-
-
-
1
1
-
-
-
-
-
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-
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134962
Godson
Action of the single-stranded ...
Aspergillus oryzae
Biochim. Biophys. Acta
308
59-67
1973
-
-
-
-
-
-
-
-
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1
1
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1
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1
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-
-
-
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3
-
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-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
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1
1
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-
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1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134960
Sutton
A crude nuclease preparation s ...
Aspergillus oryzae
Biochim. Biophys. Acta
240
522-531
1971
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-
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1
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1
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1
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-
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2
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-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
1
-
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-
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-
1
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
134959
Ando
A nuclease specific for heat d ...
Aspergillus oryzae
Biochim. Biophys. Acta
114
158-168
1966
-
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-
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1
-
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1
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1
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1
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1
-
-
-
-
-
3
-
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-
-
1
1
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-
-
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-
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-
-
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-
-
-
1
-
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-
1
-
1
-
-
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1
-
-
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3
-
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-
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-
1
1
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-