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Literature summary for 3.1.30.1 extracted from

  • Shishido, K; Habuka, N.
    Purification of S1 nuclease to homogeneity and its chemical, physical and catalytic properties (1986), Biochim. Biophys. Acta, 884, 215-218.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme contains 3 zinc atoms Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Aspergillus oryzae
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein large number of mannose residues Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
Aspergillus oryzae adenosine + phosphate
-
?
3'-CMP + H2O
-
Aspergillus oryzae cytosine + phosphate
-
?
3'-dAMP + H2O only little activity Aspergillus oryzae 3'-deoxyadenosine + phosphate
-
?
3'-GMP + H2O
-
Aspergillus oryzae guanosine + phosphate
-
?
DNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
ssDNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Synonyms

Synonyms Comment Organism
S1 nuclease
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 no inactivation by heating at 55°C, partial inactivation at 65°C, importance of high content of hydrophobic amino acids and zinc atoms for thermostability postulated Aspergillus oryzae