BRENDA - Enzyme Database
show all sequences of 3.1.3.B8

Mannosylglucosylglycerate biosynthesis in the deep-branching phylum Planctomycetes: characterization of the uncommon enzymes from Rhodopirellula baltica

Cunha, S.; dAvo, A.F.; Mingote, A.; Lamosa, P.; da Costa, M.S.; Costa, J.; Sci. Rep. 3, 2378 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli; expression in Escherichia coli
Rhodopirellula baltica
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
2 mM, no residual activity
Rhodopirellula baltica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7.5, 25C
Rhodopirellula baltica
0.22
-
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7, 25C
Rhodopirellula baltica
6.16
-
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7.5, 40C
Rhodopirellula baltica
6.16
-
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7, 40C
Rhodopirellula baltica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+
Rhodopirellula baltica
Mg2+
highest activity with 10 mM Mg2+; strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+. Maximum activity in presence of 10 mM Mg2+
Rhodopirellula baltica
Ni2+
; strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+
Rhodopirellula baltica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35900
-
x * 35900, calculated
Rhodopirellula baltica
36000
-
SDS-PAGE
Rhodopirellula baltica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodopirellula baltica
Q7UIS7
-
-
Rhodopirellula baltica
-
-
-
Rhodopirellula baltica DSM 10527
Q7UIS7
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.013
-
pH 7.5, 25C
Rhodopirellula baltica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
732910
Rhodopirellula baltica
2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
-
-
?
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
732910
Rhodopirellula baltica DSM 10527
2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
-
-
?
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
732910
Rhodopirellula baltica
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
-
-
?
additional information
enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase
732910
Rhodopirellula baltica
?
-
-
-
-
additional information
enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase
732910
Rhodopirellula baltica DSM 10527
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 35900, calculated
Rhodopirellula baltica
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
;
Rhodopirellula baltica
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
-
no activity below 15C
Rhodopirellula baltica
25
-
28% of maximum activity
Rhodopirellula baltica
90
-
18% of maximum activity
Rhodopirellula baltica
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
half-life 9.6 h; half-live: 9.6 hours
Rhodopirellula baltica
40
-
half-life 5.3 h; half-live: 5.3 hours
Rhodopirellula baltica
60
-
28% activity retained
Rhodopirellula baltica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
;
Rhodopirellula baltica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
9
-
Rhodopirellula baltica
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rhodopirellula baltica
calculated
-
5.7
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Rhodopirellula baltica
expression in Escherichia coli
Rhodopirellula baltica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
2 mM, no residual activity
Rhodopirellula baltica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7.5, 25C
Rhodopirellula baltica
0.22
-
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7, 25C
Rhodopirellula baltica
6.16
-
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7.5, 40C
Rhodopirellula baltica
6.16
-
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate
pH 7, 40C
Rhodopirellula baltica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+
Rhodopirellula baltica
Mg2+
highest activity with 10 mM Mg2+
Rhodopirellula baltica
Mg2+
strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+. Maximum activity in presence of 10 mM Mg2+
Rhodopirellula baltica
Ni2+
-
Rhodopirellula baltica
Ni2+
strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+
Rhodopirellula baltica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35900
-
x * 35900, calculated
Rhodopirellula baltica
36000
-
SDS-PAGE
Rhodopirellula baltica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.013
-
pH 7.5, 25C
Rhodopirellula baltica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
732910
Rhodopirellula baltica
2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
-
-
?
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
732910
Rhodopirellula baltica DSM 10527
2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
-
-
?
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
732910
Rhodopirellula baltica
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
-
-
?
additional information
enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase
732910
Rhodopirellula baltica
?
-
-
-
-
additional information
enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase
732910
Rhodopirellula baltica DSM 10527
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 35900, calculated
Rhodopirellula baltica
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Rhodopirellula baltica
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
-
no activity below 15C
Rhodopirellula baltica
25
-
28% of maximum activity
Rhodopirellula baltica
90
-
18% of maximum activity
Rhodopirellula baltica
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
half-live: 9.6 hours
Rhodopirellula baltica
25
-
half-life 9.6 h
Rhodopirellula baltica
40
-
half-live: 5.3 hours
Rhodopirellula baltica
40
-
half-life 5.3 h
Rhodopirellula baltica
60
-
28% activity retained
Rhodopirellula baltica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Rhodopirellula baltica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
9
-
Rhodopirellula baltica
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rhodopirellula baltica
calculated
-
5.7
Other publictions for EC 3.1.3.B8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732910
Cunha
Mannosylglucosylglycerate bios ...
Rhodopirellula baltica, Rhodopirellula baltica DSM 10527
Sci. Rep.
3
2378
2013
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1
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1
4
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3
2
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8
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1
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5
1
1
3
3
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1
1
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1
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2
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-
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-
1
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4
-
5
2
-
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-
1
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5
1
2
3
5
-
2
1
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1
-
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708933
Fernandes
Two alternative pathways for t ...
Petrotoga mobilis
J. Bacteriol.
192
1624-1633
2010
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1
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1
3
-
4
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1
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1
-
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1
-
-
-
3
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5
-
1
1
2
-
1
1
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-
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1
-
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-
-
-
1
-
3
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4
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1
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1
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3
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5
-
1
1
2
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1
1
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