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Literature summary for 3.1.3.93 extracted from

  • Torabinejad, J.; Donahue, J.L.; Gunesekera, B.N.; Allen-Daniels, M.J.; Gillaspy, G.E.
    VTC4 is a bifunctional enzyme that affects myoinositol and ascorbate biosynthesis in plants (2009), Plant Physiol., 150, 951-961.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.93

Inhibitors

Inhibitors Comment Organism Structure
D-inositol 3-phosphate substrate inhibition above 0.7 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.107
-
 beta-L-galactose 1-phosphate pH 7.5, 22°C Arabidopsis thaliana
0.191
-
 D-inositol 3-phosphate pH 7.5, 22°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 3fold activation, optimum concentration 3-4 mM Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33100
-
 x * 39700, SDS-PAGE, x * 33100, calculated Arabidopsis thaliana
39700
-
 x * 39700, SDS-PAGE, x * 33100, calculated Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M8S8 bifunctional L-galactose 1-phosphate phosphatase/myo-inositol monophosphatase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + H2O 19% of the activity with myo-inositol Arabidopsis thaliana alpha-D-glucose + phosphate
-
 ?
beta-L-galactose 1-phosphate + H2O 166-240% of the activity with myo-inositol Arabidopsis thaliana L-galactose + phosphate
-
 ?
D-galactose 1-phosphate + H2O 16.6% of the activity with myo-inositol Arabidopsis thaliana D-galactose + phosphate
-
 ?
D-inositol 3-phosphate + H2O
-
 Arabidopsis thaliana D-inositol + phosphate
-
 ?
glycerol 2-phosphate + H2O 52% of the activity with myo-inositol Arabidopsis thaliana glycerol + phosphate
-
 ?
additional information Fru 1-phosphate, Fru 1,6-bisphosphate, Glc 6-phosphate, D-alpha-glycerophosphate, sorbitol 6-phosphate, and myo-inositol 2-V show little to no hydrolysis in assays Arabidopsis thaliana ?
-
 ?
myo-inositol 1-phosphate + H2O
-
 Arabidopsis thaliana myo-inositol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 39700, SDS-PAGE, x * 33100, calculated Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
VTC4
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function knockout lines of isoform VTC4 exhibit small perturbations in abscisic acid, salt, and cold responses. Less myo-inositol and ascorbate accumulate in these mutants Arabidopsis thaliana