Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in crude cytosols glucose-6-phosphatase activity has a positive correlation with counting factor accumulation. Molecules greater than 10 kDa from cells with low counting factor activity significantly increase activity of glucose-6-phosphatase in wild-type microsomes. In smlA- and wild-type cells, detergent treatment with Triton X-100 to allow direct access of D-glucose 6-phosphate to the catalytic subunit does not increase enzymatic activity. In low counting factor crude microsomes, detergent treatment increases glucose-6-phosphatase activity | Dictyostelium discoideum | |
additional information | secreted multipolypeptide complex called counting factor regulates one or more proteins greater than 10000 Da in crude cytosol that affect microsome-associated glucose-6-phosphatase activity | Dictyostelium discoideum |
Application | Comment | Organism |
---|---|---|
medicine | crucial enzyme involved in glucose homeostasis | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of glucose-6-phosphatase in crude microsomes from cells with high, normal, or low counting factor activity has a negative correlation with the amount of counting factor present in these cell lines. Molecules greater than 10 kDa from wild-type cells strongly repress activity of glucose-6-phosphatase in wild-type microsomes | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-glucose 6-phosphate | cleared lysate of countin- cells with low activity of counting factor | Dictyostelium discoideum | |
0.1 | - |
D-glucose 6-phosphate | crude cytosol of cells with low counting factor | Dictyostelium discoideum | |
0.1 | - |
D-glucose 6-phosphate | crude cytosol of countin- cells with low activity of counting factor | Dictyostelium discoideum | |
0.1 | - |
D-glucose 6-phosphate | crude microsome of countin- cells with low activity of counting factor | Dictyostelium discoideum | |
0.18 | - |
D-glucose 6-phosphate | crude cytosol of wild-type | Dictyostelium discoideum | |
0.4 | - |
D-glucose 6-phosphate | crude cytosol of smlA- cells | Dictyostelium discoideum | |
0.4 | - |
D-glucose 6-phosphate | crude microsome of cells with disruption in smlA gene resulting in oversecretion of counting factor | Dictyostelium discoideum | |
0.65 | - |
D-glucose 6-phosphate | crude cytosol of wild-type cells | Dictyostelium discoideum | |
0.65 | - |
D-glucose 6-phosphate | crude microsome of wild-type | Dictyostelium discoideum | |
1.2 | - |
D-glucose 6-phosphate | crude cytosol of cells with disruption in smlA gene resulting in oversecretion of counting factor | Dictyostelium discoideum | |
2.1 | - |
D-glucose 6-phosphate | cleared lysate of wild-type | Dictyostelium discoideum | |
2.4 | - |
D-glucose 6-phosphate | ccleared lystae of cells with disruption in smlA gene resulting in oversecretion of counting factor | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | activity of glucose-6-phosphatase in crude cytosol is positively correlated with the amount of secreted multipolypeptide complex called counting factor | Dictyostelium discoideum | 5829 | - |
microsome | activity of glucose-6-phosphatase in crude microsome is negatively correlated with the amount of secreted multipolypeptide complex called counting factor | Dictyostelium discoideum | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | microsome | Dictyostelium discoideum | - |
additional information | Ax4 cell, HDB7YA cell (smlA- cell), HDB2B/4 cell (cells with low counting factor) | Dictyostelium discoideum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + H2O | - |
Dictyostelium discoideum | D-glucose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucose-6-phosphatase | - |
Dictyostelium discoideum |