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Literature summary for 3.1.3.9 extracted from

  • Clottes, E.; Middleditch, C.; Burchell, A.
    Rat liver glucose-6-phosphatase system: light scattering and chemical characterization (2002), Arch. Biochem. Biophys., 408, 33-41.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-nitro-5-thiocyano-benzoic acid
-
Rattus norvegicus
4-maleimidylstilbene-2,2'-disulfonic acid
-
Rattus norvegicus
5-((((iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid
-
Rattus norvegicus
DTNB
-
Rattus norvegicus
NEM
-
Rattus norvegicus
p-hydroxy mercury-phenylsulfonate
-
Rattus norvegicus
p-hydroxy mercurybenzoic acid
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
Rattus norvegicus 5789
-
microsome
-
Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + H2O Rattus norvegicus the enzyme is required for the final steps of glycogenolysis and gluconeogenesis D-glucose + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information chemical modification of thiol groups belonging to proteins involved in the enzyme system by DTNB, NTCB and IAEDANS decrease the enzyme activity, the incubation with phosphate preventes the loss of activity, the addition of 2-mercaptoethanol allows a 50% restoration of the initial activity, however chemical modification of thiol groups by pHMPS, pHMB, AMSDS and NEM decrease the enzyme activity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + H2O
-
Rattus norvegicus D-glucose + phosphate
-
?
D-glucose 6-phosphate + H2O the enzyme is required for the final steps of glycogenolysis and gluconeogenesis Rattus norvegicus D-glucose + phosphate
-
?

Subunits

Subunits Comment Organism
More the enzyme is multiprotein system, the transmembrane catalytic subunit and the D-glucose 6-phosphate translocase have reactive thiol groups facing the cytosol Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
NEM
-
Rattus norvegicus
0.5
-
4-maleimidylstilbene-2,2'-disulfonic acid
-
Rattus norvegicus
0.6
-
DTNB
-
Rattus norvegicus
0.8
-
5-((((iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid
-
Rattus norvegicus
2
-
2-nitro-5-thiocyano-benzoic acid
-
Rattus norvegicus