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Literature summary for 3.1.3.89 extracted from

  • Proudfoot, M.; Kuznetsova, E.; Brown, G.; Rao, N.N.; Kitagawa, M.; Mori, H.; Savchenko, A.; Yakunin, A.F.
    General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli. Biochemical Characterization of SurE, YfbR, and Yjjg (2004), J. Biol. Chem., 279, 54687-54694.
    View publication on PubMed
Search for more literature for 3.1.3.89

Inhibitors

Inhibitors Comment Organism Structure
ADP 0.2 mM, 21% residual activity Escherichia coli
ATP 0.2 mM, 18% residual activity Escherichia coli
CTP 0.2 mM, 29% residual activity Escherichia coli
dATP 0.2 mM, 15% residual activity Escherichia coli
dCTP 0.2 mM, 19% residual activity Escherichia coli
dGTP 0.2 mM, 13% residual activity Escherichia coli
dTTP 0.2 mM, 15% residual activity Escherichia coli
GTP 0.2 mM, 28% residual activity Escherichia coli
ITP 0.2 mM, 29% residual activity Escherichia coli
TTP 0.2 mM, 14% residual activity Escherichia coli
UTP 0.2 mM, 26% residual activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 5'-dTMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.012
-
 5'-dAMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.017
-
 5'-dIMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.02
-
 5'-dUMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.036
-
 5'-dCMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.047
-
 5'-dGMP presence of Co2+, pH 8.0, 37°C Escherichia coli
2.09
-
 4-nitrophenyl phosphate presence of Co2+, pH 8.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM Escherichia coli
Cu2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+ Escherichia coli
Mn2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 and tetramer, 3 * 24000, SDS-PAGE Escherichia coli
24000
-
 and trimer, 4 * 24000, SDS-PAGE Escherichia coli
71300
-
 and above 100000, gel filtration Escherichia coli
100000
-
 and 71300, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?
5'-dAMP + H2O
-
 Escherichia coli deoxyadenosine + phosphate
-
 ?
5'-dCMP + H2O
-
 Escherichia coli deoxycytidine + phosphate
-
 ?
5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
5'-dIMP + H2O
-
 Escherichia coli deoxyinosine + phosphate
-
 ?
5'-dTMP + H2O
-
 Escherichia coli thymidine + phosphate
-
 ?
5'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
additional information enzyme is strictly specific to deoxyribonucleoside 5'-monophosphates and does not hydrolyze ribonucleotides or deoxyribonucleoside 3'-monophosphates. It has similar levels of activity against all tested deoxyribonucleoside 5'-monophosphates Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
tetramer and trimer, 4 * 24000, SDS-PAGE Escherichia coli
trimer and tetramer, 3 * 24000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
YfbR
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 5'-dTMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.2
-
 5'-dGMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.23
-
 5'-dCMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.23
-
 5'-dUMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.24
-
 5'-dIMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.3
-
 5'-dAMP presence of Co2+, pH 8.0, 37°C Escherichia coli
0.56
-
 4-nitrophenyl phosphate presence of Co2+, pH 8.0, 37°C Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.27
-
 4-nitrophenyl phosphate presence of Co2+, pH 8.0, 37°C Escherichia coli
4.22
-
 5'-dGMP presence of Co2+, pH 8.0, 37°C Escherichia coli
6.42
-
 5'-dCMP presence of Co2+, pH 8.0, 37°C Escherichia coli
11.6
-
 5'-dUMP presence of Co2+, pH 8.0, 37°C Escherichia coli
14.1
-
 5'-dIMP presence of Co2+, pH 8.0, 37°C Escherichia coli
21.3
-
 5'-dTMP presence of Co2+, pH 8.0, 37°C Escherichia coli
25.6
-
 5'-dAMP presence of Co2+, pH 8.0, 37°C Escherichia coli