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Literature summary for 3.1.3.84 extracted from

  • Saikatendu, K.S.; Joseph, J.S.; Subramanian, V.; Clayton, T.; Griffith, M.; Moy, K.; Velasquez, J.; Neuman, B.W.; Buchmeier, M.J.; Stevens, R.C.; Kuhn, P.
    Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1’’-phosphate dephosphorylation by a conserved domain of nsP3 (2005), Structure, 13, 1665-1675.
    View publication on PubMed
Search for more literature for 3.1.3.84

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DL41 Severe acute respiratory syndrome-related coronavirus

Crystallization (Commentary)

Crystallization (Comment) Organism
nanodroplet vapor diffusion method, using 1.5 M sodium malonate (pH 7.0) Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0527
-
 ADP-ribose 1''-phosphate in 50 mM Tris (pH 7.5), 1 mM MgCl2, 0.1 mM sodium azide, temperature not specified in the publication Severe acute respiratory syndrome-related coronavirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19520
-
 calculated from amino acid sequence Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
 Tor2
-

Purification (Commentary)

Purification (Comment) Organism
ultracentrifugation, Co2+-Talon resin column chromatography, and Poros HQ column chromatography Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-ribose 1''-phosphate + H2O
-
 Severe acute respiratory syndrome-related coronavirus ADP-ribose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ADRP
-
 Severe acute respiratory syndrome-related coronavirus
Appr-1''-p phosphatase
-
 Severe acute respiratory syndrome-related coronavirus
nonstructural protein 3
-
 Severe acute respiratory syndrome-related coronavirus
nsP3
-
 Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0865
-
 ADP-ribose 1''-phosphate in 50 mM Tris (pH 7.5), 1 mM MgCl2, 0.1 mM sodium azide, temperature not specified in the publication Severe acute respiratory syndrome-related coronavirus

pI Value

Organism Comment pI Value Maximum pI Value
Severe acute respiratory syndrome-related coronavirus calculated from amino acid sequence
-
 6.9