BRENDA - Enzyme Database
show all sequences of 3.1.3.81

Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase

Takeuchi, M.; Harigai, M.; Momohara, S.; Ball, E.; Abe, J.; Furuichi, K.; Kamatani, N.; Gene 399, 174-180 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells; expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
NEM
inhibition of isozyme DPPL1; inhibition of isozyme DPPL2
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.041
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
0.104
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
isozyme DPPL1 is Mg2+-independent; isozyme DPPL2 is Mg2+-independent
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q5VZY2
DPPL2; isozyme DPPL2
-
Homo sapiens
Q8NEB5
DPPL1; isozyme DPPL1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
A-549 cell
-
Homo sapiens
-
blood vessel
synovium containing, specific expression of isozyme DPPL2
Homo sapiens
-
brain
isozyme DPPL2
Homo sapiens
-
endothelial cell
isozyme DPPL2
Homo sapiens
-
endothelium
specific expression of DPPL2, expression in endothelial cell lines, overview
Homo sapiens
-
IM-9 cell
-
Homo sapiens
-
JURKAT cell
-
Homo sapiens
-
kidney
isozyme DPPL2
Homo sapiens
-
MCF-7 cell
-
Homo sapiens
-
additional information
DPPL1 is ubiquitously expressed in various tissues and cells, overview; DPPL2 mRNA is restricted to several tissues, expression in cancer cell lines, overview
Homo sapiens
-
PC-3 cell
-
Homo sapiens
-
plasma cell
specific expression of isozyme DPPL2
Homo sapiens
-
T-lymphocyte
specific expression of isozyme DPPL2
Homo sapiens
-
testis
isozyme DPPL2
Homo sapiens
-
THP-1 cell
-
Homo sapiens
-
WiDr cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL1
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL2
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at; assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at; assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells
Homo sapiens
expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NEM
inhibition of isozyme DPPL2
Homo sapiens
NEM
inhibition of isozyme DPPL1
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.041
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
0.104
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
isozyme DPPL2 is Mg2+-independent
Homo sapiens
additional information
isozyme DPPL1 is Mg2+-independent
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
A-549 cell
-
Homo sapiens
-
blood vessel
synovium containing, specific expression of isozyme DPPL2
Homo sapiens
-
brain
isozyme DPPL2
Homo sapiens
-
endothelial cell
isozyme DPPL2
Homo sapiens
-
endothelium
specific expression of DPPL2, expression in endothelial cell lines, overview
Homo sapiens
-
IM-9 cell
-
Homo sapiens
-
JURKAT cell
-
Homo sapiens
-
kidney
isozyme DPPL2
Homo sapiens
-
MCF-7 cell
-
Homo sapiens
-
additional information
DPPL2 mRNA is restricted to several tissues, expression in cancer cell lines, overview
Homo sapiens
-
additional information
DPPL1 is ubiquitously expressed in various tissues and cells, overview
Homo sapiens
-
PC-3 cell
-
Homo sapiens
-
plasma cell
specific expression of isozyme DPPL2
Homo sapiens
-
T-lymphocyte
specific expression of isozyme DPPL2
Homo sapiens
-
testis
isozyme DPPL2
Homo sapiens
-
THP-1 cell
-
Homo sapiens
-
WiDr cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL1
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL2
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Homo sapiens
Other publictions for EC 3.1.3.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
679998
Takeuchi
Cloning and characterization o ...
Homo sapiens
Gene
399
174-180
2007
-
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1
-
-
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1
2
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1
-
-
-
2
-
-
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-
-
16
-
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2
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1
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-
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1
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2
-
-
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2
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2
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2
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-
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17
-
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2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
682952
Carman
Roles of phosphatidate phospha ...
Saccharomyces cerevisiae
Trends Biochem. Sci.
31
694-699
2006
-
-
-
-
-
-
3
-
3
1
-
1
-
1
-
-
-
-
-
-
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1
1
-
-
-
-
-
-
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-
-
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3
-
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3
1
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1
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-
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680628
Han
Vacuole membrane topography of ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae W303-1A
J. Biol. Chem.
279
5338-5345
2004
1
-
1
-
-
-
1
-
3
-
-
2
-
14
-
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1
1
-
-
2
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3
1
1
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1
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1
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1
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1
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3
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2
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1
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2
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3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
678372
Oshiro
Diacylglycerol pyrophosphate p ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1635
1-9
2003
1
-
1
-
4
-
7
-
1
1
1
2
-
1
-
-
1
1
-
-
-
-
3
2
-
-
-
-
1
-
-
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1
-
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1
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1
-
-
4
-
-
7
1
-
1
1
1
2
-
-
-
1
-
-
-
-
3
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
680624
Oshiro
Regulation of the yeast DPP1-e ...
Saccharomyces cerevisiae
J. Biol. Chem.
278
31495-31503
2003
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
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-
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-
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2
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-
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-
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-
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-
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1
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1
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-
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1
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-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
680613
Han
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
10126-10133
2001
-
-
1
-
1
-
1
-
1
-
1
1
-
2
-
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-
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-
-
-
-
1
1
1
-
-
-
1
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1
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1
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1
-
1
1
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1
-
1
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
680612
Oshiro
Regulation of the DPP1-encoded ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
40887-40896
2000
1
-
1
-
-
-
3
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
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1
-
1
1
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1
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1
3
1
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1
1
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-
-
-
-
-
-
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2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
678120
Toke
Mutagenesis of the phosphatase ...
Saccharomyces cerevisiae
Biochemistry
38
14606-14613
1999
-
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1
-
3
-
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1
-
-
-
1
-
1
-
-
-
-
-
-
5
-
2
1
1
-
3
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
5
-
2
1
1
-
3
-
1
-
-
-
-
-
-
-
-
-
680606
Toke
Isolation and characterization ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae W303-1A
J. Biol. Chem.
273
3278-3284
1998
-
-
1
-
1
-
-
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1
1
1
2
-
13
-
-
-
-
-
-
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2
2
1
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-
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1
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-
-
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1
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1
-
-
-
-
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1
1
1
2
-
-
-
-
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134878
Carman
Phosphatidate phosphatases and ...
Escherichia coli, Mus musculus, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1348
45-55
1997
-
-
1
-
1
-
5
1
1
4
1
5
-
3
-
-
1
-
-
-
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7
4
-
-
-
-
1
-
-
-
-
-
-
-
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1
-
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1
-
-
5
-
1
1
4
1
5
-
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1
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-
-
7
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
680603
Dillon
Mammalian Mg2+-independent pho ...
Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
272
10361-10366
1997
1
-
-
-
-
-
6
2
2
2
-
4
-
2
-
-
-
-
-
2
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6
-
2
-
-
-
2
-
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-
1
-
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1
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-
-
-
-
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6
1
2
2
2
-
4
-
-
-
-
-
2
-
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6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
682447
Riedel
-
Metabolism of diacylglycerol p ...
Catharanthus roseus
Plant Sci.
128
1-10
1997
-
-
-
-
-
-
6
-
2
2
-
1
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1
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4
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3
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1
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-
-
2
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-
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6
-
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2
2
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1
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-
-
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4
-
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
680601
Wu
Purification and characterizat ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae MATa ade5
J. Biol. Chem.
271
1868-1876
1996
3
-
-
-
-
-
10
1
1
-
1
2
-
2
-
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1
-
-
-
1
-
4
1
1
-
-
2
1
-
-
-
4
-
-
3
-
-
-
-
-
-
-
10
4
1
1
-
1
2
-
-
-
1
-
-
1
-
4
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
680602
Dillon
The Escherichia coli pgpB gene ...
Escherichia coli
J. Biol. Chem.
271
30548-30553
1996
1
-
1
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1
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2
-
-
1
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1
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1
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-
-
-
-
-
-
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2
-
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1
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1
1
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1
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1
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1
2
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1
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1
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2
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1
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