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Literature summary for 3.1.3.8 extracted from
- Enhancing the thermal resistance of a novel acidobacteria-derived phytase by engineering of disulfide bridges (2016), J. Microbiol. Biotechnol., 26, 1717-1722 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured bacterium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T101C/A229C/S307C/V352C/M364C/F398C | enhancing the thermal resistance of the acidobacteria-derived phytase by engineering of disulfide bridges, construction of engineered mutant rPhyA6DB by site-directed mutagenesis, overview | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
uncultured bacterium |  |
| Ca2+ | slight inhibition | uncultured bacterium | |
| Cu2+ | - |
uncultured bacterium | |
| Fe2+ | slight inhibition | uncultured bacterium | |
| Fe3+ | - |
uncultured bacterium | |
| Hg2+ | strong inhibition | uncultured bacterium | |
| Mn2+ | - |
uncultured bacterium | |
| Pb2+ | - |
uncultured bacterium | |
| Zn2+ | - |
uncultured bacterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.382 | - |
myo-inositol hexakisphosphate | recombinant wild-type enzyme, pH 2.5, 40°C | uncultured bacterium | |
| 0.405 | - |
myo-inositol hexakisphosphate | recombinant mutant enzyme, pH 2.5, 40°C | uncultured bacterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight activation | uncultured bacterium | |
| Mg2+ | slight activation | uncultured bacterium | |
| Na+ | slight activation | uncultured bacterium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | uncultured bacterium | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
isolated from a soil metagenome | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography followed by two steps of gel filtration to 95% purity | uncultured bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1792 | - |
purified recombinant enzyme, pH 2.5, 37°C | uncultured bacterium |
| 1844 | - |
purified recombinant enzyme, pH 2.5, 40°C | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | uncultured bacterium | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45741, mass spectrometry | uncultured bacterium |
| More | three-dimensional structural model, overview | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAP phytase | - |
uncultured bacterium |
| More | cf. EC 3.1.3.26 | uncultured bacterium |
| PhyA | - |
uncultured bacterium |
| phytase | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
purified recombinant His-tagged wild-type enzyme, pH 2.5, half-life is 2 h, and for mutant rPhyA6DB 5.4 h | uncultured bacterium |
| 48 | - |
melting temperature, recombinant His-tagged enzyme | uncultured bacterium |
| 60 | - |
purified recombinant His-tagged wild-type enzyme, pH 7.0, half-life is 5.2 min, and for mutant rPhyA6DB 14.5 min | uncultured bacterium |
| 80 | - |
purified recombinant His-tagged wild-type enzyme, pH 7.0, half-life is 2.3 min, and for mutant rPhyA6DB 7.0 min | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
recombinant enzyme | uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 5 | activity range of wild-type and mutant enzyme, profile overview | uncultured bacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
purified recombinant His-tagged wild-type enzyme, 37°C, half-life is 2 h (for mutant rPhyA6DB 5.4 h) | uncultured bacterium |
| 7 | - |
purified recombinant His-tagged wild-type enzyme, at 60°C the half-life is 5.2 min (for mutant rPhyA6DB 14.5 min), at 80°C the half-life is 2.3 min (for mutant rPhyA6DB 7.0 min) | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | threedimensional structural model, overview | uncultured bacterium |
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