Any feedback?
Literature summary for 3.1.3.8 extracted from
- N-terminal domain of the beta-propeller phytase of Pseudomonas sp. FB15 plays a role for retention of low-temperature activity and catalytic efficiency (2018), Enzyme Microb. Technol., 117, 84-90 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the His-tagged full-length enzyme and its N- and C-terminal domains in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and thermodynamics of recombinant full-length enzyme and C-terminal domain, overview | Pseudomonas sp. | |
| 0.856 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged C-terminal domain | Pseudomonas sp. |  |
| 0.858 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged full-length enzyme | Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | CaCl2 is required for activity, and the optimal concentration of CaCl2 for the full-length enzyme is 4 mM, the isolated C-terminal domain is most active at 3 mM | Pseudomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | Pseudomonas sp. | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | Pseudomonas sp. FB15 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | A0A1S6WME7 | isolated from foreshore soil on the coast of Busan, Korea | - |
| Pseudomonas sp. FB15 | A0A1S6WME7 | isolated from foreshore soil on the coast of Busan, Korea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged full-length enzyme and its N- and C-terminal domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity | Pseudomonas sp. | ? | - |
? | |
| additional information | the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity | Pseudomonas sp. FB15 | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Pseudomonas sp. | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Pseudomonas sp. FB15 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-propeller phytase | - |
Pseudomonas sp. |
| myo-inositol hexakisphosphate phosphohydrolase | - |
Pseudomonas sp. |
| phytase | - |
Pseudomonas sp. |
| PSphy | - |
Pseudomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | purified recombinant enzyme, over 80% activity remaining within this range, thermal stability of recobinant enzyme PSphy decreases sharply above 40°C in the presence of CaCl2, whereas it remains stable up to 60°C in its absence | Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.67 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged C-terminal domain | Pseudomonas sp. | |
| 20.39 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged full-length enzyme | Pseudomonas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified recombinant enzyme, over 60% activity remaining within this range | Pseudomonas sp. |
| 8 | - |
purified recombinant enzyme, completely stable at | Pseudomonas sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | activity and catalytic efficiency at low temperature are reduced in a recombinant protein from which the N-terminal domain is removed | Pseudomonas sp. |
| additional information | the N-terminal domain is required for the low temperature activity and high catalytic efficiency of PSphy | Pseudomonas sp. |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.95 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged C-terminal domain | Pseudomonas sp. | |
| 23.76 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged full-length enzyme | Pseudomonas sp. | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
