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Literature summary for 3.1.3.8 extracted from

  • Sato, V.S.; Jorge, J.A.; Guimaraes, L.H.
    Characterization of a thermotolerant phytase produced by Rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source (2016), Appl. Biochem. Biotechnol., 179, 610-624 .
    View publication on PubMed
Search for more literature for 3.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
additional information poor effect by EDTA at 1-2 mM Rhizopus microsporus

Application

Application Comment Organism
additional information phytases show great potential for application in different sectors such as in animal nutrition, human nutrition, aquaculture, and pharmacology. These enzymes are considered as a green feed additive that can be used to neutralize the antinutritional effects of phytate, thereby increasing the bioavailability of phosphorus and other minerals. They also contribute to the reduction of environmental pollution by phosphorus Rhizopus microsporus

Inhibitors

Inhibitors Comment Organism Structure
arsenate
-
 Rhizopus microsporus
Co2+ 45% inhibition at 2 mM Rhizopus microsporus
Cu2+ 11.5% inhibition at 2 mM Rhizopus microsporus
KCl 50% inhibition at 2 mM Rhizopus microsporus
additional information poor effect by EDTA at 1-2 mM Rhizopus microsporus
potassium phosphate complete inhibition at 1-5 mM Rhizopus microsporus
Sodium phosphate almost complete inhibition at 0.25-1.0 mM Rhizopus microsporus
Zn2+ 85% inhibition at 2 mM Rhizopus microsporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 4-nitrophenyl phosphate pH 5.0, 45°C Rhizopus microsporus
0.72
-
 myo-inositol hexakisphosphate pH 4.5, 45°C Rhizopus microsporus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhizopus microsporus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 43% at 2 mM Rhizopus microsporus
Ca2+ activates about 9% at 2 mM Rhizopus microsporus
Fe2+ activates about 10% at 0.4 mM Rhizopus microsporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Rhizopus microsporus
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus microsporus
-
 var. microsporus, isolated from Brazilian soil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 4.18fold by anion and cation exchange chromatography Rhizopus microsporus

Source Tissue

Source Tissue Comment Organism Textmining
biofilm thermotolerant phytase is produced by Rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source. The enzyme production is strictly regulated by the phosphorus content with optimal production at 0.5 mM of sodium phytate and KH2PO4 Rhizopus microsporus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2285
-
 purified enzyme, pH 4.5, 50°C Rhizopus microsporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Rhizopus microsporus 4-nitrophenol + phosphate
-
 ?
additional information the phytase demonstrates high substrate specificity for sodium phytate. The enzyme also hydrolyzes 4-nitrophenyl phosphate, ATP, AMPc, glucose 6-phosphate, glucose 1-phosphate, and UDPG Rhizopus microsporus ?
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Rhizopus microsporus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O i.e. phytate Rhizopus microsporus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 35400, extracellular enzyme, SDS-PAGE Rhizopus microsporus

Synonyms

Synonyms Comment Organism
myo-inositol hexakisphosphate phosphohydrolase
-
 Rhizopus microsporus
RMPhy1
-
 Rhizopus microsporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rhizopus microsporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 over 50% of maximal activity within this range, profile overview Rhizopus microsporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 purified enzyme, highly stable at Rhizopus microsporus
60
-
 purified enzyme, half-life is 115 min Rhizopus microsporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Rhizopus microsporus

pH Range

pH Minimum pH Maximum Comment Organism
2 9 over 70% of maximal activity within this range, profile overview Rhizopus microsporus

Expression

Organism Comment Expression
Rhizopus microsporus phytase production by Rhizopus microsporus var. microsporus biofilm is stimulated by the phosphorus source in the medium up

General Information

General Information Comment Organism
evolution on the basis of the site at which phytate dephosphorization begins, phytases can be grouped into three classes: 3-phytases (EC 3.1.3.8), 6-phytases (EC 3.1.3.26), and 5-phytases (EC 3.1.3.72). The fungal phytases are included in the 3-phytases class, in which the dephosphorization starts at the third phosphate group. Because of their catalytic mechanism, the fungal phytases belong to the histidine acid phosphatase class Rhizopus microsporus
physiological function phytases (myo-inositol hexakisphosphate phosphohydrolases) are enzymes that compose a special class of phosphatases. Phytases catalyze the hydrolysis of the phosphomonoester bonds of phytate (salts of myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate), releasing lower forms of myo-inositol and inorganic phosphates. Regulation of enzymatic synthesis by phosphate is coordinated by the PHO pathwa Rhizopus microsporus