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Literature summary for 3.1.3.8 extracted from
- Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation (2013), Biotechnol. Lett., 35, 1669-1676.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q258N | substitution introduced to enhance the glycosylation activity upon expression in Pichia pastoris | Escherichia coli |
| Q258N/Q349N | substitutions introduced to enhance the glycosylation activity upon expression in Pichia pastoris. Mutant displays 40% enhancement in thermostability compared to wild-type | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
myo-inositol hexakisphosphate | mutant Q258N/Q349N, pH 4.5, 37°C | Escherichia coli |  |
| 0.48 | - |
myo-inositol hexakisphosphate | wild-type, pH 4.5, 37°C | Escherichia coli | |
| 0.52 | - |
myo-inositol hexakisphosphate | mutant Q258N, pH 4.5, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE, wild-type | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | after treatment with EndoH, protein gives one band of 50 kDa in SDS-PAGE | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3137 | - |
mutant Q258N/Q349N, pH 4.5, 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE, wild-type | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AppA | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 67.5 | - |
melting temperature, wild-type | Escherichia coli |
| 72.2 | - |
melting temperature, mutant Q258N/Q349N | Escherichia coli |
| 85 | - |
10 min, 60% residual activity for mutant Q258N/Q349N, 5 min, 30% residual activiy for wild-type | Escherichia coli |
| 100 | - |
10 min, 40% residual activity for mutant Q258N/Q349N | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2660 | - |
myo-inositol hexakisphosphate | mutant Q258N, pH 4.5, 37°C | Escherichia coli | |
| 3058 | - |
myo-inositol hexakisphosphate | mutant Q258N/Q349N, pH 4.5, 37°C | Escherichia coli | |
| 3397 | - |
myo-inositol hexakisphosphate | wild-type, pH 4.5, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
both wild-typ and mutants Q258N and Q258N/Q349N | Escherichia coli |
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