Literature summary for 3.1.3.8 extracted from

Yao, M.; Lu, W.; Chen, T.; Wang, W.; Fu, Y.; Yang, B.; Liang, A.
Effect of metals ions on thermostable alkaline phytase from Bacillus subtilis YCJS isolated from soybean rhizosphere soil (2014), Ann. Microbiol., 64, 1123-1131.

Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.36	-	myo-inositol hexakisphosphate	pH 7.4, 37°C, presence of Ca2+	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required, assay in presence of 1.5 mM. The removal of Ca2+ causes a change in the CD-spectrum. The addition of Ca2+ to the apoenzyme results in an increase in the mean residue ellipticity, indicating a recovery of beta-sheet elements	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47500	-	x * 47500, SDS-PAGE	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	H9C9P0	subsp. spizizenii	-

Renatured (Commentary)

Renatured (Comment)	Organism
metal-depleted apoenzyme can be restored by Ca2+, Sr2+,Co2+, Ba2+, Cu2+, La3+, Ni+, Tb2+, and Mn2+ to 44.1%, 29.8%, 36.7%, 26.9%, 1.9%, 30.0%, 34.2%, 8.3%, and 34.9% of its reference activity, respectively	Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
14	-	pH 7.4, 37°C, presence of Ca2+	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol hexakisphosphate + H2O	-	Bacillus subtilis	myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 47500, SDS-PAGE	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
47.8	-	melting temperature	Bacillus subtilis
62.4	-	melting temperature, presence of Ca2+	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
25.4	-	myo-inositol hexakisphosphate	pH 7.4, 37°C, presence of Ca2+	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Bacillus subtilis

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Release 2023.1 (January 2023)