Cloned (Comment) | Organism |
---|---|
gene phyC, phylogenetic tree, expression in Pichia pastoris strain GS115 under the control of AOX1 promoter. The recombinant PhyCm, driven by the Saccharomyces cerevisiae alpha-mating factor, is secreted into culture medium | Bacillus licheniformis |
General Stability | Organism |
---|---|
supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm, overview | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 25% inhibition at 1 mM, 44% inhibition at 5 mM | Bacillus licheniformis | |
Cd2+ | 92% inhibition at 1 mM, 98% inhibition at 5 mM | Bacillus licheniformis | |
Cu2+ | 32% inhibition at 1 mM, 49% inhibition at 5 mM | Bacillus licheniformis | |
DTT | 6% inhibition at 5 mM | Bacillus licheniformis | |
EDTA | 94% inhibition at 1 mM, 100% inhibition at 5 mM | Bacillus licheniformis | |
Li+ | 10% inhibition at 1 mM, 13% inhibition at 5 mM | Bacillus licheniformis | |
Mg2+ | 18% inhibition at 1 mM, 27% inhibition at 5 mM | Bacillus licheniformis | |
Mn2+ | 49% inhibition at 1 mM, 67% inhibition at 5 mM | Bacillus licheniformis | |
PMSF | 8% inhibition at 1 mM, 10% inhibition at 5 mM | Bacillus licheniformis | |
Zn2+ | 32% inhibition at 1 mM, 40% inhibition at 5 mM | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes at 1 mM, activates 20% at 1 mM, 12% at 5 mM | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | A4UU76 | gene phyC | - |
Bacillus licheniformis ZJ-6 | A4UU76 | gene phyC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 3.3fold from culture supernatant of Pichia pastoris strain GS115 by ammonium sulfate fractionation and gel filtration | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.7 | - |
purifed recombinant enzyme, pH 7.5, 60°C | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
neutral phytase | - |
Bacillus licheniformis |
PhyCm | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant PhyCm | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant PhyCm | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm | Bacillus licheniformis |
2 | 9 | 37°C, 1 h, over 80% activity remaining of the purified recombinant PhyCm within the range of pH 5.0-9.0, profile, overveiw | Bacillus licheniformis |