Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.8 extracted from

  • Dionisio, G.; Madsen, C.; Holm, P.; Welinder, K.; Jorgensen, M.; Stoger, E.; Arcalis, E.; Brinch-Pedersen, H.
    Cloning and characterization of purple acid phosphatase phytases from wheat, barley, maize, and rice (2011), Plant Physiol., 156, 1087-1100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Triticum aestivum
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Hordeum vulgare
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Triticum aestivum
additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Hordeum vulgare
additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Zea mays
0.035
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a1, pH 5.0, 36°C Triticum aestivum
0.036
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a, pH 5.0, 36°C Hordeum vulgare
0.045
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b1, pH 5.0, 36°C Triticum aestivum
0.046
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b2, pH 5.0, 36°C Hordeum vulgare
0.048
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates isozyme b1 about 5fold at 10 mM Triticum aestivum
Mn2+ activates isozyme a1 12fold at 10 mM Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Triticum aestivum
66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Hordeum vulgare
66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Triticum aestivum
-
? + phosphate
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Hordeum vulgare
-
? + phosphate
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Zea mays
-
? + phosphate
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Triticum aestivum Bob white SH 98 26
-
? + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare C4PKL2 isozyme a; Golden Promise, isozyme a
-
Hordeum vulgare C4PKL4 isozyme b2; Golden Promise, isozyme b2
-
Triticum aestivum C4PKK7 isozyme a1; isozyme a1
-
Triticum aestivum C4PKK9 isozyme b1; isozyme b1
-
Triticum aestivum Bob white SH 98 26 C4PKK7 isozyme a1; isozyme a1
-
Triticum aestivum Bob white SH 98 26 C4PKK9 isozyme b1; isozyme b1
-
Zea mays C4PKL6 isozyme b; isozyme b
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 7 N-glycosylation sites Zea mays
glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites Hordeum vulgare
glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 9 N-glycosylation sites Triticum aestivum

Purification (Commentary)

Purification (Comment) Organism
soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration Zea mays
soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration Triticum aestivum
soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
additional information identification of wheat phytase in mature and germinating grains by tandem mass spectrometry analysis Triticum aestivum
-
seed
-
Zea mays
-
seed quantitative reverse transcription PCR enzyme expression analysis in developing grains Triticum aestivum
-
seed quantitative reverse transcription PCR enzyme expression analysis in developing grains Hordeum vulgare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of recombinant isozymes, overview Triticum aestivum ?
-
?
additional information substrate specificity of recombinant isozymes, overview Hordeum vulgare ?
-
?
additional information substrate specificity of the recombinant isozyme, overview Zea mays ?
-
?
additional information substrate specificity of recombinant isozymes, overview Triticum aestivum Bob white SH 98 26 ?
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Triticum aestivum ? + phosphate
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Hordeum vulgare ? + phosphate
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Zea mays ? + phosphate
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Triticum aestivum Bob white SH 98 26 ? + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Triticum aestivum
monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Hordeum vulgare
monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
PAP
-
Triticum aestivum
PAP
-
Hordeum vulgare
PAP
-
Zea mays
PAP type I
-
Triticum aestivum
PAP type I
-
Hordeum vulgare
PAP type I
-
Zea mays
PAPhys
-
Triticum aestivum
PAPhys
-
Hordeum vulgare
PAPhys
-
Zea mays
purple acid phosphatase
-
Triticum aestivum
purple acid phosphatase
-
Hordeum vulgare
purple acid phosphatase
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
assay at Triticum aestivum
36
-
assay at Hordeum vulgare
36
-
assay at Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
248
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Zea mays
253
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b2, pH 5.0, 36°C Hordeum vulgare
260
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a, pH 5.0, 36°C Hordeum vulgare
270
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b1, pH 5.0, 36°C Triticum aestivum
279
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a1, pH 5.0, 36°C Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Triticum aestivum
5
-
assay at Hordeum vulgare
5
-
assay at Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
517
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Zea mays
550
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b2, pH 5.0, 36°C Hordeum vulgare
600
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b1, pH 5.0, 36°C Triticum aestivum
722
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a, pH 5.0, 36°C Hordeum vulgare
796
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a1, pH 5.0, 36°C Triticum aestivum