Cloned (Comment) | Organism |
---|---|
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Triticum aestivum |
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Hordeum vulgare |
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Triticum aestivum | |
additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Hordeum vulgare | |
additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Zea mays | |
0.035 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a1, pH 5.0, 36°C | Triticum aestivum | |
0.036 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a, pH 5.0, 36°C | Hordeum vulgare | |
0.045 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b1, pH 5.0, 36°C | Triticum aestivum | |
0.046 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b2, pH 5.0, 36°C | Hordeum vulgare | |
0.048 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates isozyme b1 about 5fold at 10 mM | Triticum aestivum | |
Mn2+ | activates isozyme a1 12fold at 10 mM | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Triticum aestivum |
66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Hordeum vulgare |
66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Triticum aestivum | - |
? + phosphate | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Hordeum vulgare | - |
? + phosphate | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Zea mays | - |
? + phosphate | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Triticum aestivum Bob white SH 98 26 | - |
? + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | C4PKL2 | isozyme a; Golden Promise, isozyme a | - |
Hordeum vulgare | C4PKL4 | isozyme b2; Golden Promise, isozyme b2 | - |
Triticum aestivum | C4PKK7 | isozyme a1; isozyme a1 | - |
Triticum aestivum | C4PKK9 | isozyme b1; isozyme b1 | - |
Triticum aestivum Bob white SH 98 26 | C4PKK7 | isozyme a1; isozyme a1 | - |
Triticum aestivum Bob white SH 98 26 | C4PKK9 | isozyme b1; isozyme b1 | - |
Zea mays | C4PKL6 | isozyme b; isozyme b | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 7 N-glycosylation sites | Zea mays |
glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites | Hordeum vulgare |
glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 9 N-glycosylation sites | Triticum aestivum |
Purification (Comment) | Organism |
---|---|
soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration | Zea mays |
soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration | Triticum aestivum |
soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | identification of wheat phytase in mature and germinating grains by tandem mass spectrometry analysis | Triticum aestivum | - |
seed | - |
Zea mays | - |
seed | quantitative reverse transcription PCR enzyme expression analysis in developing grains | Triticum aestivum | - |
seed | quantitative reverse transcription PCR enzyme expression analysis in developing grains | Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of recombinant isozymes, overview | Triticum aestivum | ? | - |
? | |
additional information | substrate specificity of recombinant isozymes, overview | Hordeum vulgare | ? | - |
? | |
additional information | substrate specificity of the recombinant isozyme, overview | Zea mays | ? | - |
? | |
additional information | substrate specificity of recombinant isozymes, overview | Triticum aestivum Bob white SH 98 26 | ? | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Triticum aestivum | ? + phosphate | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Hordeum vulgare | ? + phosphate | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Zea mays | ? + phosphate | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Triticum aestivum Bob white SH 98 26 | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Triticum aestivum |
monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Hordeum vulgare |
monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Triticum aestivum |
PAP | - |
Hordeum vulgare |
PAP | - |
Zea mays |
PAP type I | - |
Triticum aestivum |
PAP type I | - |
Hordeum vulgare |
PAP type I | - |
Zea mays |
PAPhys | - |
Triticum aestivum |
PAPhys | - |
Hordeum vulgare |
PAPhys | - |
Zea mays |
purple acid phosphatase | - |
Triticum aestivum |
purple acid phosphatase | - |
Hordeum vulgare |
purple acid phosphatase | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
assay at | Triticum aestivum |
36 | - |
assay at | Hordeum vulgare |
36 | - |
assay at | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
248 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Zea mays | |
253 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b2, pH 5.0, 36°C | Hordeum vulgare | |
260 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a, pH 5.0, 36°C | Hordeum vulgare | |
270 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b1, pH 5.0, 36°C | Triticum aestivum | |
279 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a1, pH 5.0, 36°C | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Triticum aestivum |
5 | - |
assay at | Hordeum vulgare |
5 | - |
assay at | Zea mays |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
517 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Zea mays | |
550 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b2, pH 5.0, 36°C | Hordeum vulgare | |
600 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b1, pH 5.0, 36°C | Triticum aestivum | |
722 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a, pH 5.0, 36°C | Hordeum vulgare | |
796 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a1, pH 5.0, 36°C | Triticum aestivum |