Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris strain GS115, methanol induction, the recombinant enzyme is secreted | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | typical Michaelis-Menten kinetics | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes the recombinant and the native enzyme | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, deglycosylated recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31097 | 3-phytase | - |
Bacillus subtilis VTT E-68013 | O31097 | 3-phytase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is N-glycosylated | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
purified recombinant deglycosylated enzyme, pH 7.5, 37°C | Bacillus subtilis |
8.9 | - |
recombinant enzyme secreted from heterologously expressing Pichia pastoris, pH 5.5, 37°C | Bacillus subtilis |
11 | - |
purified recombinant enzyme, pH 7.5, 37°C | Bacillus subtilis |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, deglycosylated recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
PhyC | - |
Bacillus subtilis |
phytase C | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | activity range, deglycosylated recombinant enzyme | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant and native enzymes, 5 mM CaCl2, pH 5.5 or pH 7.5, stable | Bacillus subtilis |
80 | - |
deglycosylated native enzyme, 5 mM CaCl2, 10 min, 68% of initial activity remaining at pH 7.5, 36% at pH 5.5 | Bacillus subtilis |
80 | - |
deglycosylated recombinant enzyme, 5 mM CaCl2, 10 min, 85% of initial activity remaining at pH 7.5, 11% at pH 5.5 | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | assay at | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | activity range, deglycosylated recombinant enzyme | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is resistant to shrimp digestive enzymes and porcine trypsin | Bacillus subtilis |