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Literature summary for 3.1.3.8 extracted from

  • Guerrero-Olazaran, M.; Rodri­guez-Blanco, L.; Carreon-Trevino, J.; Gallegos-Lopez, J.; Viader-Salvado, J.
    Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme (2010), Appl. Environ. Microbiol., 76, 5601-5608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris strain GS115, methanol induction, the recombinant enzyme is secreted Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information typical Michaelis-Menten kinetics Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizes the recombinant and the native enzyme Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, deglycosylated recombinant enzyme, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31097 3-phytase
-
Bacillus subtilis VTT E-68013 O31097 3-phytase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is N-glycosylated Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
purified recombinant deglycosylated enzyme, pH 7.5, 37°C Bacillus subtilis
8.9
-
recombinant enzyme secreted from heterologously expressing Pichia pastoris, pH 5.5, 37°C Bacillus subtilis
11
-
purified recombinant enzyme, pH 7.5, 37°C Bacillus subtilis

Subunits

Subunits Comment Organism
? x * 39000, deglycosylated recombinant enzyme, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
PhyC
-
Bacillus subtilis
phytase C
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity range, deglycosylated recombinant enzyme Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
recombinant and native enzymes, 5 mM CaCl2, pH 5.5 or pH 7.5, stable Bacillus subtilis
80
-
deglycosylated native enzyme, 5 mM CaCl2, 10 min, 68% of initial activity remaining at pH 7.5, 36% at pH 5.5 Bacillus subtilis
80
-
deglycosylated recombinant enzyme, 5 mM CaCl2, 10 min, 85% of initial activity remaining at pH 7.5, 11% at pH 5.5 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 assay at Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
2 9 activity range, deglycosylated recombinant enzyme Bacillus subtilis

General Information

General Information Comment Organism
additional information the enzyme is resistant to shrimp digestive enzymes and porcine trypsin Bacillus subtilis