Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Penicillium parvum |
Crystallization (Comment) | Organism |
---|---|
recombinant phytase contains the active site RHGXRXP and HD sequence motifs, a large alpha/beta-domain and a small alpha-domain | Penicillium parvum |
General Stability | Organism |
---|---|
enzyme is resistant to pepsin | Penicillium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 50% inhibition | Penicillium parvum | |
Phenylmethylsulfonylfluoride | 1 mM, 17.7% of initial activity | Penicillium parvum | |
sodium dodecylsulfate | 0.5 mM, 0.35% of initial activity | Penicillium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
myo-inositol hexakisphosphate | sodium salt, pH 5.5, 50°C | Penicillium parvum | |
0.59 | - |
myo-inositol hexakisphosphate | calcium salt, pH 5.5, 50°C | Penicillium parvum | |
10.1 | - |
4-nitrophenyl phosphate | pH 5.5, 50°C | Penicillium parvum | |
114.8 | - |
myo-inositol hexakisphosphate | potassium salt, pH 5.5, 50°C | Penicillium parvum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Penicillium parvum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 112% of initial activity | Penicillium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium parvum | - |
- |
- |
Penicillium parvum BCC1769 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Penicillium parvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Penicillium parvum | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Penicillium parvum BCC1769 | 4-nitrophenol + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Penicillium parvum | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Penicillium parvum BCC1769 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium parvum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, complete loss of activity | Penicillium parvum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.8 | - |
4-nitrophenyl phosphate | pH 5.5, 50°C | Penicillium parvum | |
103 | - |
myo-inositol hexakisphosphate | sodium salt, pH 5.5, 50°C | Penicillium parvum | |
117 | - |
myo-inositol hexakisphosphate | calcium salt, pH 5.5, 50°C | Penicillium parvum | |
134 | - |
myo-inositol hexakisphosphate | potassium salt, pH 5.5, 50°C | Penicillium parvum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Penicillium parvum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | - |
Penicillium parvum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
88.8 | - |
4-nitrophenyl phosphate | pH 5.5, 50°C | Penicillium parvum | |
173.7 | - |
myo-inositol hexakisphosphate | potassium salt, pH 5.5, 50°C | Penicillium parvum | |
177.9 | - |
myo-inositol hexakisphosphate | sodium salt, pH 5.5, 50°C | Penicillium parvum | |
198.9 | - |
myo-inositol hexakisphosphate | calcium salt, pH 5.5, 50°C | Penicillium parvum |