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Literature summary for 3.1.3.8 extracted from
- Improvement of Yersinia frederiksenii phytase performance by a single amino acid substitution (2009), Biotechnol. Bioeng., 103, 857-864.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Yersinia frederiksenii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S51A | optimum temperature and pH similar to wild-type | Yersinia frederiksenii |
| S51D | optimum temperature and pH similar to wild-type | Yersinia frederiksenii |
| S51I | shift in optimal pH to 5.0. Compared with wild-type, mutant S51T shows higher specific activity, greater activity over pH 2.0-5.5, and increased thermal and acid stability | Yersinia frederiksenii |
| S51K | optimum temperature and pH similar to wild-type | Yersinia frederiksenii |
| S51T | shift in optimal pH to 4.5. Specific activity of S51T at pH 4.5 is 5.5fold higher than the corresponding specific activity of wild-type | Yersinia frederiksenii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45900 | - |
x * 46000, SDS-PAGE, x * 45900, calculated | Yersinia frederiksenii |
| 46000 | - |
x * 46000, SDS-PAGE, x * 45900, calculated | Yersinia frederiksenii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yersinia frederiksenii | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 428 | - |
pH 2.5, 37°C | Yersinia frederiksenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | sodium salt | Yersinia frederiksenii | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE, x * 45900, calculated | Yersinia frederiksenii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AppA | - |
Yersinia frederiksenii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Yersinia frederiksenii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
2 min, pH 2.5, complete loss of activity for wild-type. Mutants S51I and S51T retain about 80% of activity | Yersinia frederiksenii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
- |
Yersinia frederiksenii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 2 | 1 h, 37°C, unstable | Yersinia frederiksenii |
| 2 | 3 | 1 h, 37°C, loss of 60% of activity | Yersinia frederiksenii |
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Release 2023.1 (January 2023)
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