Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | slight stimulation | Aspergillus ficuum | |
EDTA | slight activation at 1-10 mM | Aspergillus ficuum |
Application | Comment | Organism |
---|---|---|
agriculture | the phytase has the potential to be useful as an animal feed supplement | Aspergillus ficuum |
nutrition | the phytase has the potential to be useful as an animal feed supplement | Aspergillus ficuum |
Cloned (Comment) | Organism |
---|---|
- |
Aspergillus ficuum |
General Stability | Organism |
---|---|
enzyme shows strong resistance toward pepsin and trypsin | Aspergillus ficuum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect: Ca2+, Mg2+, Mn2+, Zn2+ | Aspergillus ficuum | |
additional information | no activation or inhibition by cations K+, Ca2+, Mg2+, Mn2+, Zn2+, Cd2+, Cu2+, Pb2+, Hg2+, Fe3+, and Al3+ | Aspergillus ficuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65500 | - |
gel filtration | Aspergillus ficuum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phytate + H2O | Aspergillus ficuum | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus ficuum | - |
- |
- |
Aspergillus ficuum NTG-23 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 71.5fold by anion exchange chromatography, cation exchange chromatography, and gel filtration | Aspergillus ficuum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus ficuum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
37°C, pH 2.5 | Aspergillus ficuum |
150.1 | - |
purified native enzyme, pH 2.5, 37°C | Aspergillus ficuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-glycerophosphate + H2O | 127% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | 254% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | 254% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | 177% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | ? + phosphate | - |
? | |
AMP + H2O | 173% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | adenosine + phosphate | - |
? | |
AMP + H2O | 173% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | adenosine + phosphate | - |
? | |
ATP + H2O | 256% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | ? + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | 228% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | D-fructose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | 228% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | D-fructose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 190% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 190% of the activity with myo-inositol hexakisphosphate | Aspergillus ficuum NTG-23 | D-glucose + phosphate | - |
? | |
additional information | the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate | Aspergillus ficuum | ? | - |
? | |
additional information | the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate | Aspergillus ficuum NTG-23 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | sodium salt | Aspergillus ficuum | myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
phytate + H2O | - |
Aspergillus ficuum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65500, SDS-PAGE | Aspergillus ficuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
67 | - |
- |
Aspergillus ficuum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | purified native enzyme, stable at 60°C, no obvious loss of activity upon incubation at 70°C for 20 min | Aspergillus ficuum |
70 | - |
20 min, no loss of activity | Aspergillus ficuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.3 | - |
- |
Aspergillus ficuum |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme shows strong resistance toward pepsin and trypsin | Aspergillus ficuum |