Application | Comment | Organism |
---|---|---|
nutrition | use of Yersinia rohdei phytase as an attractive additive to animal feed. Compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and a potential commercial phytase from Yersinia intermedia, the Yersinia rohdei phytase is more resistant to pepsin, retains more activity under gastric conditions, and releases more inorganic phosphorus from soybean meal under simulated gastric conditions | Yersinia rohdei |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Yersinia rohdei |
expression in Pichia pastoris | Yersinia pestis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.196 | - |
myo-inositol hexakisphosphate | pH 4.5, 37°C | Yersinia pestis | |
0.206 | - |
myo-inositol hexakisphosphate | pH 4.5, 37°C | Yersinia rohdei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45200 | - |
x * 46000, SDS-PAGE, x * 45200, calculated | Yersinia pestis |
46000 | - |
x * 46000, SDS-PAGE, x * 45200, calculated | Yersinia pestis |
46000 | - |
x * 46000, SDS-PAGE, x * 46100, calculated | Yersinia rohdei |
46100 | - |
x * 46000, SDS-PAGE, x * 46100, calculated | Yersinia rohdei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | Q7CIZ7 | - |
- |
Yersinia rohdei | B4X9S4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Yersinia rohdei |
recombinant protein | Yersinia pestis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2344 | - |
pH 4.5, 37°C | Yersinia pestis |
2456 | - |
pH 4.5, 37°C | Yersinia rohdei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Yersinia rohdei | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Yersinia pestis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE, x * 45200, calculated | Yersinia pestis |
? | x * 46000, SDS-PAGE, x * 46100, calculated | Yersinia rohdei |
Synonyms | Comment | Organism |
---|---|---|
AppA | - |
Yersinia pestis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Yersinia rohdei |
55 | - |
- |
Yersinia pestis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
2 min, 35% residual activity | Yersinia pestis |
80 | - |
2 min, 50% residual activity | Yersinia rohdei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2283 | - |
myo-inositol hexakisphosphate | pH 4.5, 37°C | Yersinia pestis | |
2531 | - |
myo-inositol hexakisphosphate | pH 4.5, 37°C | Yersinia rohdei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Yersinia rohdei |
4.5 | - |
- |
Yersinia pestis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 5.5 | - |
Yersinia pestis |
2 | 5.5 | more than 80% of maximum activity | Yersinia rohdei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.6 | - |
myo-inositol hexakisphosphate | pH 4.5, 37°C | Yersinia pestis | |
12.3 | - |
myo-inositol hexakisphosphate | pH 4.5, 37°C | Yersinia rohdei |