Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X33 | Debaryomyces castellii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
myo-inositol hexakisphosphate | native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C | Debaryomyces castellii | |
0.532 | - |
myo-inositol hexakisphosphate | recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C | Debaryomyces castellii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
SDS-PAGE, deglycosylated form | Debaryomyces castellii |
51200 | - |
deduced from amino acid sequence | Debaryomyces castellii |
52980 | - |
mass spectrometry analysis, deglycosylated form | Debaryomyces castellii |
74460 | - |
mass spectrometry analysis, glycosylated form | Debaryomyces castellii |
77000 | - |
SDS-PAGE, glycosylated form | Debaryomyces castellii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Debaryomyces castellii | A2TBB4 | - |
- |
Debaryomyces castellii CBS 2923 | A2TBB4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Debaryomyces castellii |
Purification (Comment) | Organism |
---|---|
ultracentrifugation and HiPrep Phenyl FF column chromatography | Debaryomyces castellii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101 | - |
crude extract, recombinant enzyme, at 37°C | Debaryomyces castellii |
182 | - |
after 1.8fold purification, recombinant enzyme, at 37°C | Debaryomyces castellii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Debaryomyces castellii | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Debaryomyces castellii CBS 2923 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
myo-inositol hexakisphosphate 3-phosphohydrolase | - |
Debaryomyces castellii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Debaryomyces castellii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51.2 | - |
myo-inositol hexakisphosphate | recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C | Debaryomyces castellii | |
59.2 | - |
myo-inositol hexakisphosphate | native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C | Debaryomyces castellii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Debaryomyces castellii |