Literature summary for 3.1.3.8 extracted from

Ragon, M.; Neugnot-Roux, V.; Chemardin, P.; Moulin, G.; Boze, H.
Molecular gene cloning and overexpression of the phytase from Debaryomyces castellii CBS 2923 (2008), Protein Expr. Purif., 58, 275-283.

Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris strain X33	Debaryomyces castellii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.24	-	myo-inositol hexakisphosphate	native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C	Debaryomyces castellii
0.532	-	myo-inositol hexakisphosphate	recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C	Debaryomyces castellii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	SDS-PAGE, deglycosylated form	Debaryomyces castellii
51200	-	deduced from amino acid sequence	Debaryomyces castellii
52980	-	mass spectrometry analysis, deglycosylated form	Debaryomyces castellii
74460	-	mass spectrometry analysis, glycosylated form	Debaryomyces castellii
77000	-	SDS-PAGE, glycosylated form	Debaryomyces castellii

Organism

Organism	UniProt	Comment	Textmining
Debaryomyces castellii	A2TBB4	-	-
Debaryomyces castellii CBS 2923	A2TBB4	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Debaryomyces castellii

Purification (Commentary)

Purification (Comment)	Organism
ultracentrifugation and HiPrep Phenyl FF column chromatography	Debaryomyces castellii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
101	-	crude extract, recombinant enzyme, at 37°C	Debaryomyces castellii
182	-	after 1.8fold purification, recombinant enzyme, at 37°C	Debaryomyces castellii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol hexakisphosphate + H2O	-	Debaryomyces castellii	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Debaryomyces castellii CBS 2923	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
myo-inositol hexakisphosphate 3-phosphohydrolase	-	Debaryomyces castellii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Debaryomyces castellii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
51.2	-	myo-inositol hexakisphosphate	recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C	Debaryomyces castellii
59.2	-	myo-inositol hexakisphosphate	native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C	Debaryomyces castellii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	4.5	-	Debaryomyces castellii

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Release 2023.1 (January 2023)