Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 49% inhibitory effect at 5 mM | Kodamaea ohmeri | |
Cu2+ | 17.5% inhibitory effect at 5 mM | Kodamaea ohmeri | |
Fe2+ | 25.7% inhibitory effect at 5 mM | Kodamaea ohmeri | |
Fe3+ | 19.4% inhibitory effect at 5 mM | Kodamaea ohmeri | |
Hg2+ | 99.2% inhibitory effect at 5 mM | Kodamaea ohmeri | |
iodoacetic acid | 6.3% inhibitory effect at 1 mM | Kodamaea ohmeri | |
additional information | not inhibited by 1 mM EDTA and 5 mM 1,10-phenanthroline | Kodamaea ohmeri | |
phenylglyoxal hydrate | 45.3% inhibitory effect at 5 mM | Kodamaea ohmeri | |
phenylmethylsulfonyl fluoride | 11.8% inhibitory effect at 10 mM | Kodamaea ohmeri | |
Zn2+ | 30.3% inhibitory effect at 5 mM | Kodamaea ohmeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.45 | - |
myo-inositol hexakisphosphate | 5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C | Kodamaea ohmeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 19.5% stimulatory effect at 5 mM | Kodamaea ohmeri | |
Ca2+ | 10.8% stimulatory effect at 5 mM | Kodamaea ohmeri | |
Co2+ | 25.5% stimulatory effect at 5 mM | Kodamaea ohmeri | |
K+ | 14.9% stimulatory effect at 5 mM | Kodamaea ohmeri | |
Li+ | 17.5% stimulatory effect at 5 mM | Kodamaea ohmeri | |
Mg2+ | 21.7% stimulatory effect at 5 mM | Kodamaea ohmeri | |
Mn2+ | 17.8% stimulatory effect at 5 mM | Kodamaea ohmeri | |
Na+ | 14.8% stimulatory effect at 5 mM | Kodamaea ohmeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92900 | - |
gel filtration | Kodamaea ohmeri |
98200 | - |
SDS-PAGE | Kodamaea ohmeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kodamaea ohmeri | - |
- |
- |
Kodamaea ohmeri BG3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE Sepharose Fast Flow column chromatography | Kodamaea ohmeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
114.6 | - |
supernantant, at 60°C | Kodamaea ohmeri |
823.3 | - |
after 7.2fold purification, at 60°C | Kodamaea ohmeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Kodamaea ohmeri | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Kodamaea ohmeri BG3 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 92900, gel filtration | Kodamaea ohmeri |
Synonyms | Comment | Organism |
---|---|---|
PhyA | - |
Kodamaea ohmeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Kodamaea ohmeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the residual phytase activity still maintains 96.3% of the control after treatment at 60°C for 1 h, the enzyme is inactivated rapidly at temperatures higher than 60°C and is almost inactivated at 70°C within 1 h | Kodamaea ohmeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.93 | - |
myo-inositol hexakisphosphate | 5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C | Kodamaea ohmeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Kodamaea ohmeri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | more than 94.0% of the residual activity is maintained after treatment at pH from 3.0 to 9.0 | Kodamaea ohmeri |