Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus awamori strains pGP209 and pGF11 | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
SDS-PAGE | Aspergillus niger |
70000 | - |
SDS-PAGE, wild type enzyme PyhA | Aspergillus awamori |
84000 | - |
SDS-PAGE | Aspergillus sp. |
84000 | - |
SDS-PAGE, recombinant enzyme pGP209 | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus niger | - |
variant ficuum | - |
Aspergillus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus niger |
glycoprotein | - |
Aspergillus sp. |
glycoprotein | - |
Aspergillus awamori |
Purification (Comment) | Organism |
---|---|
Sephadex G-100 gel filtration and DEAE-Sepharose CL-6B anion exchange column chromatography | Aspergillus awamori |
Renatured (Comment) | Organism |
---|---|
the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation | Aspergillus niger |
the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation | Aspergillus sp. |
the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus sp. | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus awamori | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Allzyme | commercial preparation | Aspergillus sp. |
Natuphos | commercial preparation | Aspergillus niger |
pGF11 phytase | recombinant enzyme | Aspergillus awamori |
pGP209 phytase | recombinant enzyme | Aspergillus awamori |
PhyA | - |
Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus sp. |
55 | - |
- |
Aspergillus niger |
55 | - |
recombinant enzyme pGP209 | Aspergillus awamori |
60 | - |
wild type PhyA | Aspergillus awamori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation, the activity is negligible at 70°C | Aspergillus niger |
50 | 70 | the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation, the activity is negligible at 70°C | Aspergillus sp. |
50 | 70 | the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation, the activity is negligible at 70°C | Aspergillus awamori |