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Literature summary for 3.1.3.8 extracted from

  • Martin, J.A.; Murphy, R.A.; Power, R.F.
    Purification and physico-chemical characterisation of genetically modified phytases expressed in Aspergillus awamori (2006), Biores. Technol., 97, 1703-1708.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus awamori strains pGP209 and pGF11 Aspergillus awamori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
SDS-PAGE Aspergillus niger
70000
-
SDS-PAGE, wild type enzyme PyhA Aspergillus awamori
84000
-
SDS-PAGE Aspergillus sp.
84000
-
SDS-PAGE, recombinant enzyme pGP209 Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus niger
-
variant ficuum
-
Aspergillus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus niger
glycoprotein
-
Aspergillus sp.
glycoprotein
-
Aspergillus awamori

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-100 gel filtration and DEAE-Sepharose CL-6B anion exchange column chromatography Aspergillus awamori

Renatured (Commentary)

Renatured (Comment) Organism
the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation Aspergillus niger
the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation Aspergillus sp.
the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Aspergillus sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Aspergillus awamori 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
Allzyme commercial preparation Aspergillus sp.
Natuphos commercial preparation Aspergillus niger
pGF11 phytase recombinant enzyme Aspergillus awamori
pGP209 phytase recombinant enzyme Aspergillus awamori
PhyA
-
Aspergillus awamori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus sp.
55
-
-
Aspergillus niger
55
-
recombinant enzyme pGP209 Aspergillus awamori
60
-
wild type PhyA Aspergillus awamori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation, the activity is negligible at 70°C Aspergillus niger
50 70 the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation, the activity is negligible at 70°C Aspergillus sp.
50 70 the enzyme denatures at temperatures of 50°C or above, when the temperature is reduced again the protein refolds almost entirely into a fully active, native conformation, the activity is negligible at 70°C Aspergillus awamori