Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | decreases activity below 0.1 mM or above 2 mM | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.0 mM, required for activity | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens DS11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA superflow resin column chromatography and HiLoad 16/10 phenyl Sepharose column chromatography | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol 1,2,4,5,6-pentakisphosphate + H2O | - |
Bacillus amyloliquefaciens | myo-inositol-2,4,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol 1,2,4,5,6-pentakisphosphate + H2O | - |
Bacillus amyloliquefaciens DS11 | myo-inositol-2,4,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol 2,4,5,6-tetrakisphosphate + H2O | - |
Bacillus amyloliquefaciens | myo-inositol 2,4,6-trisphosphate + phosphate | - |
? | |
myo-inositol 2,4,5,6-tetrakisphosphate + H2O | - |
Bacillus amyloliquefaciens DS11 | myo-inositol 2,4,6-trisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus amyloliquefaciens | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus amyloliquefaciens DS11 | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-propeller phytase | beta-propeller phytase has substrate specificity for myo-inositol hexakisphosphate and yields a myo-inositol trisphosphate and three phosphate groups as the final products | Bacillus amyloliquefaciens |