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Literature summary for 3.1.3.8 extracted from

  • Zhang, W.; Mullaney, E.J.; Lei, X.G.
    Adopting selected hydrogen bonding and ionic interactions from Aspergillus fumigatus phytase structure improves the thermostability of Aspergillus niger PhyA phytase (2007), Appl. Environ. Microbiol., 73, 3069-3076.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 Aspergillus niger

Protein Variants

Protein Variants Comment Organism
A58E/P65S increased activity at pH 5.5 Aspergillus niger
A58E/P65S/Q191R/T271R increased activity and improved thermostability retaining 20% greater activity after being heated at 80°C for 10 min and has a 7°C higher melting temperature than that of wild type PhyA Aspergillus niger
A58E/Q191R increased activity at pH 5.5 Aspergillus niger
A58E/Q191R/T271R increased activity at pH 5.5 Aspergillus niger
E35A no activity Aspergillus niger
E35A/R168A no activity and decreased thermostability Aspergillus niger
E35A/R168A/R248A no activity and 25% loss of thermostability Aspergillus niger
E35A/R248A no activity and decreased thermostability Aspergillus niger
R168A no activity Aspergillus niger
R168A/R248A no activity and decreased thermostability Aspergillus niger
R248A no activity Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1064
-
 myo-inositol hexakisphosphate mutant enzyme A58E/P65S/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
0.1304
-
 myo-inositol hexakisphosphate mutant enzyme A58E/P65S, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
0.154
-
 myo-inositol hexakisphosphate mutant enzyme A58E/Q191R, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
0.1623
-
 myo-inositol hexakisphosphate mutant enzyme A58E/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
0.1675
-
 myo-inositol hexakisphosphate wild type enzyme, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid metal affinity chromatography, DEAE cation-exchange chromatography, and Sephadex-100 gel filtration Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Afp
-
 Aspergillus fumigatus
PhyA
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
66.3
-
 the melting temperature of the wild type enzyme is 66.3°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
 Aspergillus niger
2.5
-
 optima at pH 2.5 and 5.5 Aspergillus fumigatus
5.5
-
-
 Aspergillus niger
5.5
-
 optima at pH 2.5 and 5.5 Aspergillus fumigatus