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Literature summary for 3.1.3.8 extracted from

  • Kim, H.W.; Kim, Y.O.; Lee, J.H.; Kim, K.K.; Kim, Y.J.
    Isolation and characterization of a phytase with improved properties from Citrobacter braakii (2003), Biotechnol. Lett., 25, 1231-1234.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 enhances activity Citrobacter braakii

General Stability

General Stability Organism
the enzyme is resistant to proteases such as trypsin or pepsin when incubated for 2 h at 37°C. Papain, elastase and pancreatin slightly decrease activity to 85%, 80% and 70%, respectively, of the original activity Citrobacter braakii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Citrobacter braakii
Fe3+
-
Citrobacter braakii
additional information not affected at concentrations up to 10 mM by: EDTA, Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ Citrobacter braakii
SDS 0.0025%, complete inactivation Citrobacter braakii
Urea 8 M, complete inactivation Citrobacter braakii
Zn2+
-
Citrobacter braakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
myo-inositol hexakisphosphate pH 4, 37°C Citrobacter braakii

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Citrobacter braakii 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected at concentrations up to 10 mM by: Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ Citrobacter braakii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, SDS-PAGE Citrobacter braakii

Organism

Organism UniProt Comment Textmining
Citrobacter braakii
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Citrobacter braakii YH-15
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
-
Citrobacter braakii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3457
-
-
Citrobacter braakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O 11% of the activity with myo-inositol hexakisphosphate Citrobacter braakii 4-nitrophenol + phosphate
-
?
additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii ?
-
?
additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii YH-15 ?
-
?
myo-inositol hexakisphosphate + H2O
-
Citrobacter braakii ? + phosphate
-
?
tetrasodium diphosphate + H2O 6% of the activity with myo-inositol hexakisphosphate Citrobacter braakii ?
-
?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Citrobacter braakii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Citrobacter braakii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 25°C: about 45% of maximal activity, 60°C: about 35% of maximal activity Citrobacter braakii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 4, 30 min, stable up to Citrobacter braakii
55
-
pH 4, 30 min, about 65% loss of activity Citrobacter braakii
60
-
pH 4, 30 min, about 90% loss of activity Citrobacter braakii
65
-
pH 4, 30 min, about 95% loss of activity Citrobacter braakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Citrobacter braakii

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5.5 about 50% of maximal activity at pH 2.5 and pH 5.5 Citrobacter braakii