Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | enhances activity | Citrobacter braakii |
General Stability | Organism |
---|---|
the enzyme is resistant to proteases such as trypsin or pepsin when incubated for 2 h at 37°C. Papain, elastase and pancreatin slightly decrease activity to 85%, 80% and 70%, respectively, of the original activity | Citrobacter braakii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Citrobacter braakii | |
Fe3+ | - |
Citrobacter braakii | |
additional information | not affected at concentrations up to 10 mM by: EDTA, Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ | Citrobacter braakii | |
SDS | 0.0025%, complete inactivation | Citrobacter braakii | |
Urea | 8 M, complete inactivation | Citrobacter braakii | |
Zn2+ | - |
Citrobacter braakii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
myo-inositol hexakisphosphate | pH 4, 37°C | Citrobacter braakii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Citrobacter braakii | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected at concentrations up to 10 mM by: Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ | Citrobacter braakii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Citrobacter braakii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter braakii | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Citrobacter braakii YH-15 | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Purification (Comment) | Organism |
---|---|
- |
Citrobacter braakii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3457 | - |
- |
Citrobacter braakii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | 11% of the activity with myo-inositol hexakisphosphate | Citrobacter braakii | 4-nitrophenol + phosphate | - |
? | |
additional information | less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate | Citrobacter braakii | ? | - |
? | |
additional information | less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate | Citrobacter braakii YH-15 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Citrobacter braakii | ? + phosphate | - |
? | |
tetrasodium diphosphate + H2O | 6% of the activity with myo-inositol hexakisphosphate | Citrobacter braakii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Citrobacter braakii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Citrobacter braakii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | 25°C: about 45% of maximal activity, 60°C: about 35% of maximal activity | Citrobacter braakii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 4, 30 min, stable up to | Citrobacter braakii |
55 | - |
pH 4, 30 min, about 65% loss of activity | Citrobacter braakii |
60 | - |
pH 4, 30 min, about 90% loss of activity | Citrobacter braakii |
65 | - |
pH 4, 30 min, about 95% loss of activity | Citrobacter braakii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Citrobacter braakii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5.5 | about 50% of maximal activity at pH 2.5 and pH 5.5 | Citrobacter braakii |