Literature summary for 3.1.3.8 extracted from

Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.; Ziegelhoffer, T.; Austin-Phillips, S.
Fungal phyA gene expressed in potato leaves produces active and stable phytase (2003), Biochem. Biophys. Res. Commun., 306, 603-609.

Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in potato leaves	Aspergillus ficuum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	recombinant enzyme is inhibited by antibody raised against a 20-mer internal peptide	Aspergillus ficuum
myo-inositol hexasulfate	0.02 mM, 90% inhibition, recombinant enzyme	Aspergillus ficuum
Phenylglyoxal	60 nM, complete inactivation, recombinant enzyme	Aspergillus ficuum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.027	-	myo-inositol hexakisphosphate	pH 5.0, 58°C, native enzyme	Aspergillus ficuum
0.124	-	myo-inositol hexakisphosphate	pH 5.0, 58°C, recombinant enzyme	Aspergillus ficuum

Organism

Organism	UniProt	Comment	Textmining
Aspergillus ficuum	-	The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the recombinant enzyme expressed in potato leaves is 15% less glycosylated than the native enzyme	Aspergillus ficuum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
179.5	-	recombinant enzyme in potato leaves	Aspergillus ficuum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Aspergillus ficuum	4-nitrophenol + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus ficuum	? + phosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
58	-	-	Aspergillus ficuum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
38	58	38°C: about 40% of maximal activity, 58°Coptimal activity, 70°C: no activity, reaction with phytate, recombinant enzyme	Aspergillus ficuum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	and a second lower optimum at pH 2.5, reaction with 4-nitrophenyl phosphate, native and recombinant enzyme	Aspergillus ficuum
5	-	reaction with phytate, native and recombinant enzyme	Aspergillus ficuum

pH Range

pH Minimum	pH Maximum	Comment	Organism
2.5	5	pH 2.5: about 45% of maximal activity, pH 5.0: about 20% of maximal activity, reaction with 4-nitrophenyl phosphate, recombinant enzyme	Aspergillus ficuum
2.5	5	pH 2.5: about 60% of maximal activity, pH 5.0: optimum, pH 6.0: no activity, reaction with phytate, recombinant enzyme	Aspergillus ficuum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00156	-	myo-inositol hexasulfate	pH 5.0, 58°C, recombinant enzyme	Aspergillus ficuum
0.0046	-	myo-inositol hexasulfate	pH 5.0, 58°C, native enzyme	Aspergillus ficuum

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Release 2023.1 (January 2023)