Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus fumigatus |
gene phyA is cloned and expressed in Pichia pastoris | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Cyclohexanedione | 2 mM, 50% loss of activity | Aspergillus fumigatus | |
myo-inositol hexasulfate | 0.01 mM, 50% inhibition | Aspergillus fumigatus | |
Phenylglyoxal | - |
Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus fumigatus | |
0.04 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus ficuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | 100000 | - |
Aspergillus fumigatus |
85000 | 100000 | - |
Aspergillus ficuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus ficuum | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Aspergillus fumigatus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Aspergillus fumigatus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | ? + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | a broad proptein band of 85000-100000 Da is observed after SDS-PAGE | Aspergillus fumigatus |
More | a broad protein band of 85000-100000 Da is observed after SDS-PAGE | Aspergillus ficuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
- |
Aspergillus fumigatus |
58 | - |
- |
Aspergillus ficuum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity | Aspergillus fumigatus |
20 | 75 | 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity, reaction with phytate | Aspergillus fumigatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
2 min, stable | Aspergillus fumigatus |
70 | - |
2 min, 42% loss of activity | Aspergillus fumigatus |
70 | - |
2 min, 42% loss of activity, irreversible | Aspergillus fumigatus |
80 | - |
2 min, 92% loss of activity | Aspergillus fumigatus |
80 | - |
2 min, 92% loss of activity, irreversible | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46 | - |
myo-inositol hexakisphosphate | - |
Aspergillus fumigatus | |
46 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus fumigatus | |
348 | - |
myo-inositol hexakisphosphate | - |
Aspergillus ficuum | |
348 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus ficuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
4-nitrophenyl phosphate as substrate | Aspergillus fumigatus |
4 | - |
reaction with 4-nitrophenyl phosphate | Aspergillus fumigatus |
5 | - |
- |
Aspergillus ficuum |
5 | - |
and a second optimum at pH 2.5 | Aspergillus ficuum |
5 | - |
myo-inositol hexakisphosphate as substrate | Aspergillus fumigatus |
5 | - |
reaction with phytate | Aspergillus fumigatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 3.0: no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, 4-nitrophenyl phosphate as substrate | Aspergillus fumigatus |
4 | 6 | pH 4.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, myo-inositol hexakisphosphate as substrate | Aspergillus fumigatus |
4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 50% of maximal activity, reaction with phytate | Aspergillus fumigatus |
4 | 5 | pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate | Aspergillus fumigatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00064 | - |
myo-inositol hexasulfate | ph 5.0, 58°C | Aspergillus fumigatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus ficuum | - |
- |
4.94 |
Aspergillus fumigatus | - |
- |
7.04 |