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Literature summary for 3.1.3.8 extracted from
- Phytase: source, preparation and exploitation (1998), Folia Microbiol. (Praha), 43, 323-338.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | addition of Aspergillus niger phytase to the flour containing wheat bran increases iron absorption in humans | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
myo-inositol hexakisphosphate | - |
Bacillus subtilis |  |
| 0.04 | - |
myo-inositol hexakisphosphate | - |
Schwanniomyces castellii | |
| 0.04 | - |
myo-inositol hexakisphosphate | strain NRRL 3135 | Aspergillus niger | |
| 0.13 | - |
myo-inositol hexakisphosphate | - |
Escherichia coli | |
| 0.15 | - |
myo-inositol hexakisphosphate | - |
Rhizopus microsporus var. oligosporus | |
| 0.25 | - |
myo-inositol hexakisphosphate | - |
Citrobacter freundii | |
| 0.3 | - |
myo-inositol hexakisphosphate | - |
Raoultella terrigena | |
| 0.35 | - |
myo-inositol hexakisphosphate | - |
Aspergillus carbonarius | |
| 0.39 | - |
myo-inositol hexakisphosphate | - |
Aspergillus niger | |
| 0.44 | - |
myo-inositol hexakisphosphate | strain 92 | Aspergillus niger | |
| 0.48 | - |
myo-inositol hexakisphosphate | strain IIIAn/8 | Aspergillus niger | |
| 0.5 | - |
myo-inositol hexakisphosphate | Bacillus subtilis var. natto | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
| extracellular | - |
Aspergillus niger | - |
- |
| extracellular | - |
Candida tropicalis | - |
- |
| extracellular | - |
Kluyveromyces marxianus | - |
- |
| extracellular | - |
Torulopsis candida | - |
- |
| extracellular | - |
Neurospora sp. | - |
- |
| extracellular | - |
Aspergillus oryzae | - |
- |
| extracellular | - |
Aspergillus terreus | - |
- |
| extracellular | - |
Aspergillus sp. | - |
- |
| extracellular | - |
Penicillium sp. | - |
- |
| extracellular | - |
Aspergillus fumigatus | - |
- |
| extracellular | - |
Penicillium camemberti | - |
- |
| extracellular | - |
Mucor sp. | - |
- |
| extracellular | - |
Aspergillus carneus | - |
- |
| extracellular | - |
Rhizopus microsporus var. oligosporus | - |
- |
| extracellular | - |
Debaryomyces castellii | - |
- |
| intracellular | - |
Escherichia coli | 5622 | - |
| intracellular | - |
Citrobacter freundii | 5622 | - |
| intracellular | - |
Raoultella terrigena | 5622 | - |
| intracellular | - |
Klebsiella sp. | 5622 | - |
| intracellular | - |
Klebsiella oxytoca | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | 38000 | Bacillus subtilis var. natto | Bacillus subtilis |
| 36500 | - |
- |
Bacillus subtilis |
| 40000 | - |
- |
Raoultella terrigena |
| 42000 | - |
- |
Escherichia coli |
| 60000 | 81000 | - |
Penicillium camemberti |
| 80000 | - |
- |
Aspergillus niger |
| 85000 | 100000 | strain NRRL3135 | Aspergillus niger |
| 100000 | - |
strain 92 | Aspergillus niger |
| 200000 | - |
- |
Aspergillus niger |
| 214000 | - |
- |
Aspergillus terreus |
| 490000 | - |
- |
Klebsiella oxytoca |
| 700000 | - |
high molecular weight form, gel filtration | Klebsiella aerogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus carbonarius | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Aspergillus carneus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus niger 92 | - |
- |
- |
| Aspergillus niger IIIAn/8 | - |
- |
- |
| Aspergillus niger NRRL 3135 | - |
- |
- |
| Aspergillus oryzae | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Aspergillus sp. | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Aspergillus terreus | - |
- |
- |
| Bacillus subtilis | - |
var. natto | - |
| Candida tropicalis | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Citrobacter freundii | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Debaryomyces castellii | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Escherichia coli | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Klebsiella aerogenes | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Klebsiella oxytoca | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Klebsiella oxytoca | - |
MO-3 | - |
| Klebsiella oxytoca MO-3 | - |
MO-3 | - |
| Klebsiella sp. | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Kluyveromyces marxianus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Mucor sp. | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Neurospora sp. | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Paramecium sp. | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Penicillium camemberti | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Penicillium sp. | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Raoultella terrigena | - |
- |
- |
| Rhizopus microsporus var. oligosporus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Saccharomyces cerevisiae | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Schwanniomyces castellii | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Torulopsis candida | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Saccharomyces cerevisiae | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Klebsiella aerogenes | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Candida tropicalis | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Kluyveromyces marxianus | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Torulopsis candida | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Citrobacter freundii | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Neurospora sp. | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus oryzae | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus terreus | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Raoultella terrigena | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus sp. | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Penicillium sp. | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Penicillium camemberti | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus carbonarius | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Klebsiella oxytoca | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Mucor sp. | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus carneus | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Rhizopus microsporus var. oligosporus | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Debaryomyces castellii | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Schwanniomyces castellii | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Paramecium sp. | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger 92 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger NRRL 3135 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger IIIAn/8 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Klebsiella oxytoca MO-3 | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus carneus |
| 45 | - |
- |
Penicillium camemberti |
| 50 | - |
- |
Aspergillus oryzae |
| 52 | - |
- |
Citrobacter freundii |
| 53 | - |
- |
Aspergillus carbonarius |
| 55 | - |
- |
Bacillus subtilis |
| 55 | - |
- |
Escherichia coli |
| 55 | - |
- |
Aspergillus niger |
| 55 | - |
- |
Klebsiella oxytoca |
| 55 | - |
- |
Rhizopus microsporus var. oligosporus |
| 58 | - |
- |
Aspergillus niger |
| 58 | - |
- |
Raoultella terrigena |
| 60 | - |
- |
Neurospora sp. |
| 60 | - |
- |
Aspergillus fumigatus |
| 60 | - |
var. natto | Bacillus subtilis |
| 70 | - |
- |
Aspergillus terreus |
| 77 | - |
- |
Klebsiella oxytoca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
and a second optimum at pH 5.0 | Citrobacter freundii |
| 3 | - |
- |
Aspergillus niger |
| 3 | - |
- |
Penicillium camemberti |
| 4.4 | - |
- |
Schwanniomyces castellii |
| 4.5 | - |
- |
Escherichia coli |
| 4.5 | - |
- |
Klebsiella aerogenes |
| 4.5 | - |
- |
Aspergillus terreus |
| 4.5 | - |
- |
Rhizopus microsporus var. oligosporus |
| 4.7 | - |
- |
Aspergillus carbonarius |
| 5 | - |
- |
Aspergillus niger |
| 5 | - |
- |
Raoultella terrigena |
| 5 | - |
- |
Aspergillus fumigatus |
| 5 | - |
and a second optimum which is lower at pH 2.7 | Citrobacter freundii |
| 5 | 6 | - |
Neurospora sp. |
| 5 | 6 | - |
Klebsiella oxytoca |
| 5.5 | - |
- |
Aspergillus niger |
| 5.5 | - |
- |
Aspergillus oryzae |
| 5.5 | - |
- |
Aspergillus terreus |
| 5.6 | - |
- |
Aspergillus carneus |
| 5.6 | - |
- |
Rhizopus microsporus var. oligosporus |
| 6 | 6.5 | - |
Aspergillus fumigatus |
| 6 | 6.5 | var. natto | Bacillus subtilis |
| 7 | 7.5 | - |
Bacillus subtilis |
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