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Literature summary for 3.1.3.8 extracted from

  • Li, J.; Hegeman, C.E.; Hanlon, R.W.; Lacy, G.H.; Denbow, D.M.; Grabau, E.A.
    Secretion of active recombinant phytase from soybean cell-suspension cultures (1997), Plant Physiol., 114, 1103-1111.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Glycine max, inserted into soybean transformation plasmid under control of constitutive and seed-specific promoters Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Storage Stability

Storage Stability Organism
4°C, stable for 2 months Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
at pH 5.5 Aspergillus niger
63
-
at pH 5.0 Aspergillus niger
66
-
at pH 4.5 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
10 min, 10% loss of the activity of the recombinant phytase, 25% loss of the activity of the fungal enzyme Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
at 58°C, and a second optimum at pH 5.5 Aspergillus niger
5.5
-
at 58°C, and a second optimum at pH 3.0 Aspergillus niger