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Literature summary for 3.1.3.8 extracted from

  • Shah, V.; Parekh, L.J.
    Phytase from Klebsiella sp. No. PG-2: Purification and properties (1990), Indian J. Biochem. Biophys., 27, 98-102.
    View publication on PubMed
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
Ag2+
-
 Klebsiella sp.
Cu2+
-
 Klebsiella sp.
F-
-
 Klebsiella sp.
Hg2+
-
 Klebsiella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 myo-inositol hexakisphosphate
-
 Klebsiella sp.
2
-
 inositol triphosphate
-
 Klebsiella sp.
2
-
 inositol monophosphate
-
 Klebsiella sp.
2
-
 inositol pentaphosphate
-
 Klebsiella sp.
2
-
 inositol tetraphosphate
-
 Klebsiella sp.
2
-
 inositol bisphosphate
-
 Klebsiella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
 gel filtration Klebsiella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella sp. inducible enzyme ?
-
 ?
additional information Klebsiella sp. PG-2 inducible enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella sp.
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Klebsiella sp.
-
 No. PG-2
-
Klebsiella sp. PG-2
-
 No. PG-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.5
-
-
 Klebsiella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O 7.6% of the activity with myo-inositol hexakisphosphate Klebsiella sp. 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 7.6% of the activity with myo-inositol hexakisphosphate Klebsiella sp. PG-2 4-nitrophenol + phosphate
-
 ?
inositol bisphosphate + H2O
-
 Klebsiella sp. ?
-
 ?
inositol bisphosphate + H2O
-
 Klebsiella sp. PG-2 ?
-
 ?
inositol monophosphate + H2O
-
 Klebsiella sp. inositol + phosphate
-
 ?
inositol monophosphate + H2O
-
 Klebsiella sp. PG-2 inositol + phosphate
-
 ?
inositol pentaphosphate + H2O
-
 Klebsiella sp. ?
-
 ?
inositol tetraphosphate + H2O
-
 Klebsiella sp. ?
-
 ?
inositol triphosphate + H2O
-
 Klebsiella sp. ?
-
 ?
additional information inducible enzyme Klebsiella sp. ?
-
 ?
additional information inducible enzyme Klebsiella sp. PG-2 ?
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Klebsiella sp. ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Klebsiella sp. PG-2 ? + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Klebsiella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable up to Klebsiella sp.
50
-
 10 min, 37% loss of activity Klebsiella sp.
60
-
 10 min, complete inactivation Klebsiella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 all inositol phosphates studied Klebsiella sp.