BRENDA - Enzyme Database
show all sequences of 3.1.3.78

Alteration of epithelial structure and function associated with PtdIns(4,5)P2 degradation by a bacterial phosphatase

Mason, D.; Mallo, G.V.; Terebiznik, M.R.; Payrastre, B.; Finlay, B.B.; Brumell, J.H.; Rameh, L.; Grinstein, S.; J. Gen. Physiol. 129, 267-283 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
involvement of phosphatidylinositol 4,5-bisphosphate phosphatase in Salmonella-induced diarrhea is explored
Salmonella enterica
Cloned(Commentary)
Commentary
Organism
by cloning SigD into a mammalian expression vector, it is introduced into intact epithelia cells by microinjection: wild-type SigD induces striking morphological and functional changes that are not mimicked by a phosphatase-deficient SigD mutant C462S
Salmonella enterica
Engineering
Amino acid exchange
Commentary
Organism
C462S
no functional or morphological changes in epithelial cells transfected with phosphatase-deficient mutant C462S
Salmonella enterica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
-
681168
Salmonella enterica
1D-myo-inositol 1,4,5,6-tetrakisphosphate + phosphate
-
-
-
?
D-myo-phosphatidylinositol 4,5-bisphosphate
-
681168
Salmonella enterica
D-myo-phosphatidylinositol 5-phosphate
-
-
-
?
additional information
evidence is provided that SigD functions as a 4’-phosphatase that dephosphorylates D-myo-phosphatidylinositol 4,5-bisphosphate to form D-myo-phosphatidylinositol 5-phosphate
681168
Salmonella enterica
?
-
-
-
-
Application (protein specific)
Application
Commentary
Organism
medicine
involvement of phosphatidylinositol 4,5-bisphosphate phosphatase in Salmonella-induced diarrhea is explored
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
by cloning SigD into a mammalian expression vector, it is introduced into intact epithelia cells by microinjection: wild-type SigD induces striking morphological and functional changes that are not mimicked by a phosphatase-deficient SigD mutant C462S
Salmonella enterica
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C462S
no functional or morphological changes in epithelial cells transfected with phosphatase-deficient mutant C462S
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
-
681168
Salmonella enterica
1D-myo-inositol 1,4,5,6-tetrakisphosphate + phosphate
-
-
-
?
D-myo-phosphatidylinositol 4,5-bisphosphate
-
681168
Salmonella enterica
D-myo-phosphatidylinositol 5-phosphate
-
-
-
?
additional information
evidence is provided that SigD functions as a 4’-phosphatase that dephosphorylates D-myo-phosphatidylinositol 4,5-bisphosphate to form D-myo-phosphatidylinositol 5-phosphate
681168
Salmonella enterica
?
-
-
-
-
Other publictions for EC 3.1.3.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715020
Guittard
Evidence for a positive role o ...
Homo sapiens
FEBS Lett.
584
2455-2460
2010
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681168
Mason
Alteration of epithelial struc ...
Salmonella enterica
J. Gen. Physiol.
129
267-283
2007
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682554
Zou
Type I phosphatidylinositol-4, ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
104
16834-16839
2007
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666740
Ungewickell
The identification and charact ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
102
18854-18859
2005
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8
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16
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4
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697201
Aleman
The amino-terminal non-catalyt ...
Salmonella enterica subsp. enterica serovar Typhimurium
Cell. Microbiol.
7
1432-1446
2005
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7
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697606
Niebuhr
Conversion of PtdIns(4,5)P(2) ...
Shigella flexneri
EMBO J.
21
5069-5078
2002
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700351
Terebiznik
Elimination of host cell PtdIn ...
Salmonella enterica subsp. enterica serovar Typhimurium
Nat. Cell Biol.
4
766-773
2002
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3
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