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Literature summary for 3.1.3.76 extracted from

  • Cronin, A.; Mowbray, S.; Duerk, H.; Homburg, S.; Fleming, I.; Fisslthaler, B.; Oesch, F.; Arand, M.
    The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase (2003), Proc. Natl. Acad. Sci. USA, 100, 1552-1557.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.76

Cloned(Commentary)

Cloned (Comment) Organism
arabinose-inducible expression of His-tagged wild-type and D9A mutant enzymes and N-terminal domains from cDNA in Escherichia coli strain BL21-AI, expression of strep-tagged enzyme from full-length gene in Escherichia coli strain XL1-Blue Homo sapiens
arabinose-inducible expression of the enzyme from full-length gene in Escherichia coli strain BL21-AI Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D9A site-directed mutagenesis, inactive mutant N-terminal domain Homo sapiens
additional information the isolated recombinant N-terminal domain is a fully active phosphatase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 20 mM Homo sapiens
EDTA complete inhibition at 20 mM Mus musculus
EDTA complete inhibition at 20 mM Rattus norvegicus
additional information no inhibition of the phosphatase activity by dicyclohexylurea and 4-fluorochalcone oxide Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens
additional information
-
 additional information kinetics Rattus norvegicus
0.24
-
 4-nitrophenyl phosphate pH 7.8, 37°C, native enzyme Rattus norvegicus
0.37
-
 4-nitrophenyl phosphate pH 7.8, 37°C, recombinant enzyme Rattus norvegicus
1.1
-
 4-nitrophenyl phosphate pH 7.8, 37°C, recombinant wild-type enzyme Homo sapiens
2.3
-
 4-nitrophenyl phosphate pH 7.8, 37°C, recombinant wild-type N-terminal domain Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus
Mg2+
-
 Homo sapiens
Mg2+ required for phosphatase activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
 male Fischer rats
-

Purification (Commentary)

Purification (Comment) Organism
from liver to homogeneity by ultracentrifugation and affinity chromatography on a benzyl thio resin using 4-fluorochalcone oxide as eluent Rattus norvegicus
recombinant His-tagged wild-type and D9A mutant enzymes and N-terminal domains from Escherichia coli strain BL21-AI by nickel affinity chromatography, recombinant strep-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by streptavidin affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Mus musculus
a
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Homo sapiens
a
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-70°C, 0.5 mg/ml purified enzyme, completely stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl phosphate + H2O
-
 Rattus norvegicus 4-methylumbelliferone + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Mus musculus 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Rattus norvegicus 4-nitrophenol + phosphate
-
 ?
additional information no activity with 4-nitrophenyl sulfate of the isolated recombinant N-terminal domain Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Mus musculus
dimer
-
 Homo sapiens
dimer
-
 Rattus norvegicus
More the phosphatase activity of the enzyme is located at the 35 kDa N-terminal part which has a alpha/beta-fold, the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity of EC 3.3.2.10, structure analysis Mus musculus
More the phosphatase activity of the enzyme is located at the 35 kDa N-terminal part which has a alpha/beta-fold, the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity of EC 3.3.2.10, structure analysis Homo sapiens
More the phosphatase activity of the enzyme is located at the 35 kDa N-terminal part which has a alpha/beta-fold, the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity of EC 3.3.2.10, structure analysis Rattus norvegicus

Synonyms

Synonyms Comment Organism
More EC 3.1.3.76 belongs to the haloacid dehalogenase superfamily of enzymes, c.f. EC 3.3.2.10 Homo sapiens
More EC 3.1.3.76 belongs to the haloacid dehalogenase superfamily of enzymes, see also EC 3.3.2.10 Mus musculus
More EC 3.1.3.76 belongs to the haloacid dehalogenase superfamily of enzymes, see also EC 3.3.2.10 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0061
-
 4-nitrophenyl phosphate pH 7.8, 37°C, recombinant wild-type enzyme Homo sapiens
0.013
-
 4-nitrophenyl phosphate pH 7.8, 37°C, recombinant wild-type N-terminal domain Homo sapiens
0.84
-
 4-nitrophenyl phosphate pH 7.8, 37°C, native enzyme Rattus norvegicus
1.61
-
 4-nitrophenyl phosphate pH 7.8, 37°C, recombinant enzyme Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Mus musculus
7.8
-
 assay at Homo sapiens
7.8
-
 assay at Rattus norvegicus