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Literature summary for 3.1.3.75 extracted from

  • Otero, L.H.; Beassoni, P.R.; Boetsch, C.; Lisa, A.T.; Domenech, C.E.
    Different effects of Mg2+ and Zn2+ on the two sites for alkylammonium compounds in Pseudomonas aeruginosa phosphorylcholine phosphatase (2011), Enzyme Res., 2011, 918283.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.75

Cloned(Commentary)

Cloned (Comment) Organism
gene pchP, expression of His-tagged enzyme in Escherichia coli strain BL21 Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Chlorocholine
-
 Pseudomonas aeruginosa
choline
-
 Pseudomonas aeruginosa
decamethonium
-
 Pseudomonas aeruginosa
hexamethonium
-
 Pseudomonas aeruginosa
additional information inhibition mechanism of alkylammonium compounds, enzyme PchP contains two sites for alkylammonium compounds: one in the catalytic site near the metal ion-phosphoester pocket, and the other in an inhibitory site responsible for the binding of the alkylammonium moiety Pseudomonas aeruginosa
phosphocholine substrate inhibition at high concentration Pseudomonas aeruginosa
Tetramethylammonium chloride
-
 Pseudomonas aeruginosa
trimethylamine
-
 Pseudomonas aeruginosa
Zn2+ Zn2+ is an activator at pH 5.0 but an reversible inhibitor at pH 7.4. Activation or inhibition of PchP by Zn2+ is caused by the transition from octahedral to tetrahedral geometry in the coordination sphere of the metal ion Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates Pseudomonas aeruginosa
Mg2+ Mg2+ is an equal activator for the enzyme at pH 5.0 and at pH 7.4. Mg2+ produces a relaxed or open conformation Pseudomonas aeruginosa
additional information Zn2+ has 1000fold stronger affinity for PchP compared to Mg2+ Pseudomonas aeruginosa
Zn2+ Zn2+ is an activator at pH 5.0 but an reversible inhibitor at pH 7.4. Activation or inhibition of PchP by Zn2+ is caused by the transition from octahedral to tetrahedral geometry in the coordination sphere of the metal ion Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphocholine + H2O Pseudomonas aeruginosa
-
 choline + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HTR2 gene pchP
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phosphorylcholine phosphatase catalyzes the hydrolysis of 4-nitrophenylphosphate Pseudomonas aeruginosa ?
-
 ?
phosphocholine + H2O
-
 Pseudomonas aeruginosa choline + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the haloacid dehalogenase superfamily Pseudomonas aeruginosa
PChP
-
 Pseudomonas aeruginosa
phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics for alkylammonium compounds trimethylamine, tetramethylammonium chloride, choline, chlorocholine, hexamethonium, and decamethonium, in presence of Zn2+ or Mg2+, overview Pseudomonas aeruginosa

General Information

General Information Comment Organism
additional information catalytic mechanism of phosphocholine with Pcho as the substrate, Mg2+ or Zn2+ as activators, and alkylammonium compounds as inhibitors, overview. Zn2+ induces a conformational change in the active center that is communicated to the inhibitory site, producing a compact or closed structure. In contrast, Mg2+ produces a relaxed or open conformation Pseudomonas aeruginosa