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Literature summary for 3.1.3.75 extracted from
- Identification, cloning, and expression of Pseudomonas aeruginosa phosphorylcholine phosphatase gene (2005), Curr. Microbiol., 50, 251-256.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is induced by growth on choline, betaine, dimethylglycine, or carnitine, choline and betaine are inhibitory at high concentrations | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PA5292, expression in Escherichia coli, expression in and complementation of a Pseudomonas aeruginosa mutant strain JUF8-00 | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Tn5-751 transposon mutagenesis is used to construct an enzyme-deficient, inactive mutant JUF8-00 of Pseudomonas aeruginosa, the mutant can be complemented by expression of the wild-type enzyme | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| betaine | 32% inhibition of wild-type enzyme in vivo at 5 mM, at lower concentrations betaine induces the enzyme expression in vivo | Pseudomonas aeruginosa |  |
| choline | 12% inhibition of wild-type enzyme in vivo at 5 mM, at lower concentrations choline induces the enzyme expression in vivo | Pseudomonas aeruginosa | |
| additional information | no inhibition by high concentrations of phosphorylcholine | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phosphocholine + H2O | Pseudomonas aeruginosa | - |
choline + phosphate | - |
? | |
| O-phosphoethanolamine + H2O | Pseudomonas aeruginosa | - |
ethanolamine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
gene PA5292 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli by chitin affinity chromatography | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + phosphate | - |
? | |
| O-phosphocholine + H2O | - |
Pseudomonas aeruginosa | choline + phosphate | - |
? | |
| O-phosphoethanolamine + H2O | - |
Pseudomonas aeruginosa | ethanolamine + phosphate | - |
? | |
| phospho-L-serine + H2O | - |
Pseudomonas aeruginosa | L-serine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PChP | - |
Pseudomonas aeruginosa |
| phosphorylcholine phosphatase | - |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at, substrates phospho-L-serine and 4-nitrophenyl phosphate | Pseudomonas aeruginosa |
| 7.4 | - |
assay at, substrates O-phosphocholine and phospho-L-serine | Pseudomonas aeruginosa |
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